Publications by authors named "Adnan Asadbeigi"
Background: Accurate detection of the BRAF V600E (1799T > A) mutation status can significantly contribute to selecting an optimal therapeutic strategy for diverse cancer types. CRISPR-based diagnostic platforms exhibit simple programming, cost-effectiveness, high sensitivity, and high specificity in detecting target sequences. The goal of this study is to develop a simple BRAF V600E mutation detection method.
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- CRISPR-Cas systems, originally used by bacteria for immunity, are being adapted to target and combat diseases caused by RNA viruses in plants and mammals.
- Researchers focused on Cas13 orthologs to effectively knockdown the foot-and-mouth disease virus (FMDV), which impacts many species worldwide due to its genetic diversity.
- The study found that various Cas13 enzymes could successfully suppress FMDV by targeting highly conserved regions of its genome, demonstrating a promising strategy for addressing other RNA viruses in animal health.
The efficiency of the CRISPR-Cas system is highly dependent on well-designed CRISPR RNA (crRNA). To facilitate the use of various types of CRISPR-Cas systems, there is a need for the development of computational tools to design crRNAs which cover different CRISPR-Cas systems with off-target analysis capability. Numerous crRNA design tools have been developed, but nearly all of them are dedicated to design crRNA for genome editing.
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