Label-free, real-time monitoring of membrane binding events at zeptomolar concentrations using frequency-locked optical microresonators.

G-protein coupled receptors help regulate cellular function and communication, and are targets of small molecule drug discovery efforts. Conventional techniques to probe these interactions require labels and large amounts of receptor to achieve satisfactory sensitivity. Here, we use frequency-locked optical microtoroids for label-free characterization of membrane interactions in vitro at zeptomolar concentrations for the kappa opioid receptor and its native agonist dynorphin A 1-13, as well as big dynorphin (dynorphin A and dynorphin B) using a supported biomimetic membrane.

Towards Early Diagnosis and Screening of Alzheimer's Disease Using Frequency Locked Whispering Gallery Mode Microtoroid Biosensors.

Alzheimer's disease (AD) is a progressive form of dementia affecting almost 55 million people worldwide. It is characterized by the abnormal deposition of amyloid plaques and neurofibrillary tangles within the brain, leading to a pathological cascade of neuron degeneration and death as well as memory loss and cognitive decline. Amyloid beta (Aβ) is an AD biomarker present in cerebrospinal fluid and blood serum and correlates with the presence of amyloid plaques and tau tangles in the brain.

Methotrexate Inhibits the Binding of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Receptor Binding Domain to the Host-Cell Angiotensin-Converting Enzyme-2 (ACE-2) Receptor.

As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus mutates, finding effective drugs becomes more challenging. In this study, we use ultrasensitive frequency locked microtoroid optical resonators in combination with screening to search for COVID-19 drugs that can stop the virus from attaching to the human angiotensin-converting enzyme 2 (hACE2) receptor in the lungs. We found 29 promising candidates that could block the binding site and selected four of them that were likely to bind very strongly.

Label-free, real-time monitoring of membrane binding events at zeptomolar concentrations using frequency-locked optical microresonators.

Binding events to elements of the cell membrane act as receptors which regulate cellular function and communication and are the targets of many small molecule drug discovery efforts for agonists and antagonists. Conventional techniques to probe these interactions generally require labels and large amounts of receptor to achieve satisfactory sensitivity. Whispering gallery mode microtoroid optical resonators have demonstrated sensitivity to detect single-molecule binding events.

Optical frequency combs in aqueous and air environments at visible to near-IR wavelengths.

The ability to detect and identify molecules at high sensitivity without the use of labels or capture agents is important for medical diagnostics, threat identification, environmental monitoring, and basic science. Microtoroid optical resonators, when combined with noise reduction techniques, have been shown capable of label-free single molecule detection; however, they still require a capture agent and prior knowledge of the target molecule. Optical frequency combs can potentially provide high precision spectroscopic information on molecules within the evanescent field of the microresonator; however, this has not yet been demonstrated in air or aqueous biological sensing.

How Fast It Can Stick: Visualizing Flow Delivery to Microtoroid Biosensors.

Sensitive and rapid biosensors are of critical importance for a variety of applications including infectious disease detection and monitoring as well as medical diagnostics and drug discovery. Whispering gallery mode microtoroid optical resonators are among the most sensitive biochemical sensors in existence. When combined with frequency-locking and data-processing techniques, these sensors have been shown to be capable of single-molecule detection in under 30 s.

Wide-angle MEMS-based imaging lidar by decoupled scan axes.

We present an optical architecture for a scanning lidar in which a digital micromirror device (DMD) is placed at an intermediate image plane in a receiver to decouple the trade-offs between scan angle, scan speed, and aperture size of the lidar's transmitter and receiver. In the architecture, the transmitter with a galvo mirror and the receiver with a DMD scan the horizontal and vertical fields of view, respectively, to enable an increased field of view of 50°, centimeter transmitter beam diameter, and video frame rate range finding captures. We present our optimized system and discuss the adjustable parameter trade-offs.

Ultrasensitive Detection of Human Chorionic Gonadotropin Using Frequency Locked Microtoroid Optical Resonators.

Clean sport competition is of significant concern to many governments and sporting organizations. Highly sensitive and rapid sensors are needed to improve the detection of performance enhancing drugs in sports as athletes take diuretics to dilute the concentration of drugs in their urine and microdose under the detectable limits of current sensors. Here we demonstrate, using frequency locked microtoroid optical resonators, a 3 orders of magnitude improvement in detection limit over the current gold standard, mass spectrometry, for the common performance enhancing drug, human chorionic gonadotropin (hCG).

Single chip lidar with discrete beam steering by digital micromirror device.

A novel method of beam steering enables a large field of view and reliable single chip light detection and ranging (lidar) by utilizing a mass-produced digital micromirror device (DMD). Using a short pulsed laser, the micromirrors' rotation is frozen in mid-transition, which forms a programmable blazed grating. The blazed grating efficiently redistributes the light to a single diffraction order, among several.