Aristapedioid: a gain of function, homeotic mutation in Drosophila melanogaster.

The isolation of gain of function mutations has allowed the identification of a number of genes which are important in the normal development of the organism. We report here the isolation and characterization of Aristapedioid, a gain of function mutation which causes a partial transformation of arista towards tarsus and the loss or decrease in size of the dorso-central and scutellar bristles. Aristapedioid is the result of a P element mediated inversion which juxtaposes unrelated DNA adjacent to Suppressor 2 of zeste, causing a gain of function mutation in that gene.

A Drosophila tissue polarity locus encodes a protein containing seven potential transmembrane domains.

The function of the frizzled (fz) locus in Drosophilia melanogaster is required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles (for example on the wild-type wing all hairs point towards the distal tip). In fz mutants it is not the structure of individual hairs and bristles that is altered, but their orientation with respect to their neighbours and the organism as a whole. Mitotic clone analysis indicates that fz has two functions in the developing wing.

Genetic interactions of the suppressor 2 of zeste region genes.

A wide variety of gain of function mutations have been induced in the Posterior Sex Comb (Psc)--Aristapedioid (Arp)--Suppressor 2 of zeste (Su(z)2) region of the second chromosome of Drosophila. This region contains at least three apparently related genes, two of which we have been studying. Psc1 has previously been used to identify Psc as a Pc group gene; however, it is a complex mutation with both gain and loss of function character.

Tissue-specific induction of murine glutathione transferase mRNAs by butylated hydroxyanisole.

Three murine liver glutathione transferase (GT, EC 2.5.1.

Directional non-cell autonomy and the transmission of polarity information by the frizzled gene of Drosophila.

The function of the frizzled (fz) locus is required for the development of a parallel array of bristles and hairs on the adult cuticle of Drosophila melanogaster. Marked fz mitotic clones from five alleles were generated and examined in the wing. Three alleles have a non-cell-autonomous hair polarity phenotype; wild-type cells distal to fz clones produce hairs that have an abnormal polarity.

Verapamil interaction with the muscarinic receptor: stereoselectivity at two sites.

Verapamil, in addition to blocking calcium channels, exhibits such "non-specific" effects on myocardium as inhibition of sodium and potassium conductances and modifications of muscarinic receptor-ligand interactions. To characterize further the effects of verapamil on the cardiac muscarinic receptor, we examined the abilities of the enantiomers of verapamil to modify the binding of the muscarinic antagonist [3H]quinuclidinyl benzilate ([3H]QNB) to purified canine sarcolemmal vesicles. Membranes were incubated with [3H]QNB and various concentrations of racemic, (+)-, or (-)- verapamil (25 or 37 degrees, pH 7.

Protein synthesis during imaginal disc pattern regulation.

We have examined the pattern of protein synthesis during wing disc pattern regulation. Although in vivo culture dramatically alters the pattern of abundant protein synthesis in wing discs, only one protein--RG38--changes specifically in response to pattern regulation. This polypeptide, previously identified as being nonuniformly distributed in wing and haltere discs, is synthesized in a graded distribution across the wing disc.

Serum glucose changes after administration of 50% dextrose solution: pre- and in-hospital calculations.

A prospective clinical trial was conducted to estimate the rise in serum glucose level after an intravenous bolus of 50 ml of 50% dextrose solution (D-50) in the emergency department setting. Fifty one subjects with altered levels of consciousness were studied. Of these, 23 patients were known diabetics, and 28 were not diabetic.

Color preferences in films for fundus photography.

Photographs that provide a colorimetrically faithful image of the original object are known to be subjectively unacceptable. Color photographic films depict a distortion of the color of the original in order to produce a subjectively acceptable reproduction. The distorsions involved may not be appropriate for the reproduction of ocular fundus photographs.

Stool examination: culture versus Gram stain.

Presented is a methodology for efficient, cost-effective collection, transport, culture, and microscopic examination of infectious diarrhea. A critical review of the literature is included, as are guidelines for workup and management of the disease complex in the emergency department.

Employee privacy: legal and research developments and implications for personnel administration.

The contemporary emphasis on privacy has created a myriad of complex personnel problems and associated approaches to handle them. These privacy-oriented problems generally involve social, ethical, legal, managerial, and even political considerations. Personnel administrators are constantly confronted with the privacy issue.

Cell-free translation of Drosophila imaginal disc mRNA.

Total RNA derived from the imaginal discs of Drosophila melanogaster was translated in vitro, and the polypeptide products electrophoresed on two-dimensional gels. In agreement with previously published examinations of imaginal disc protein synthesis and content, we can detect no reproducible differences in abundant mRNA populations between different disc types (foreleg and wing). Differences can be found, however, between imaginal discs and other tissues.

Cell proliferation and DNA replication in the imaginal wing disc of Drosophila melanogaster.

Cell proliferation in the imaginal wing disc of Drosophila has been analyzed by both pulse and chronic labeling with [3H]thymidine. We find neither spatial nor temporal variation in the fraction of S phase cells during the third instar. At or near the time of white prepupae formation the fraction of S phase cells falls sharply.

DNA replication and pattern regulation in the imaginal wing disc of Drosophila.

Imaginal disc cells of Drosophila undergo pattern regulation when part of a disc is removed by microsurgery. I have analyzed DNA replication in imaginal disc cells during pattern regulation using both pulse and chronic labeling with tritiated thymidine. Both types of experiments have shown a local increase in the fraction of labeled cells associated with pattern regulation.

Mathematical description and use in forecasting of the caries curve.

Caries curves drawn from empirical prevalences (DMFT counts) of US and Hungarian males as well as females, and British male-female combined samples were satisfactorily described by the transformed truncated Cauchy function: y = 1 + c [-1/2 + 1/pi arc tan (a x + b)]. The parameters a, b, and c, respectively, were determined by a computer with the least squares' method. Of these descriptive models, prevalences at specified ages (covered in the original reports) were read, and additionally the half-life values and maximum yearly relative increments of decay (with the age of their occurrence).

Calcium fluxes across the membrane of sarcoplasmic reticulum vesicles.

The relationship between calcium exchange across the membrane of sarcoplasmic reticulum vesicles and phosphoenzyme (EP) was examined in calcium transport reactions using a limited amount of ATP as substrate. Rapid calcium influx and efflux (approximately 385 nmol.(mg.

Synergistic effect of human relaxin and progesterone on human myometrial contractions.

In an in vitro human myometrial strip system, both relaxin and progesterone can independently decrease the amplitude of spontaneous myometrial contractions. However, progesterone and relaxin synergize in this action. Doses of relaxin and progesterone which independently are ineffective, together inhibit myometrial contraction amplitude.