Tobacco ringspot virus Found in the Cardboard Cycad (Zamia furfuracea) in Florida.

Zamia furfuracea (Zamiaceae) is native of coastal Mexico. It is a popular houseplant and easy to grow outdoors in warm climates. In November 2005, a plant of Z.

Natural Infection of Verbena and Phlox by a Recently Described Member of the Carmovirus Genus.

A novel carmovirus infecting angelonia (Angelonia angustifolia) was recently described independently by researchers in the United States, Israel, and Germany (1,2,4). Angelonia flower break virus (AnFBV) and Angelonia flower mottle virus were proposed as appropriate names for this carmovirus. The virus, causing stunting, mild leaf mottle, flower mottling, and flower breaking symptoms has been detected in naturally infected angelonia in the United States, Israel, and Germany (2,4).

Clover yellow vein virus Identified in Ammi majus in Florida.

Ammi majus L., a member of the Apiaceae and also known as large bullwort, false Queen Anne's lace, or bishop's-weed, is frequently used in the floral trade to add a lacey look to floral bouquets. A.

The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids.

A Florida isolate of Tomato spotted wilt virus (TSWV) was able to complement cell-to-cell movement of a movement-defective Tobacco mosaic virus (TMV) vector expressing the jellyfish green fluorescent protein (GFP). To test for complementation of movement in the absence of other TSWV proteins, the open reading frame for the NSm protein was expressed from TMV constructs encoding only the TMV replicase proteins. NSm was expressed from either the coat protein or movement protein subgenomic promoter, creating virus hybrids that moved cell to cell in inoculated leaves of tobacco, providing the first functional demonstration that NSm is the TSWV movement protein.

Tomato spotted wilt virus Identified in Desert Rose in Florida.

Desert rose (Adenium obesum (Forssk.) Roem. & Schult), a member of the family Apocynaceae, is characterized by fleshy stems and leaves and colorful flowers.

Determination of complete nucleotide sequence of Hibiscus latent Singapore virus: evidence for the presence of an internal poly(A) tract.

We have sequenced the complete genome of a hibiscus-infecting tobamovirus, Hibiscus latent Singapore virus (HLSV). The experimental host range of HLSV is similar to that of another distinct species of hibiscus infecting tobamovirus, Hibiscus latent Fort Pierce virus (HLFPV). The genomic structure of HLSV is similar to other tobamoviruses in general.

Physical activity among African-American girls: the role of parents and the home environment.

Objectives: African-American girls are at increased risk for becoming overweight. Increased physical activity may prevent this. This study examines measurements of girls' physical activity and associations with: BMI, parent's reported self-efficacy and support for helping daughters be active, girl's perceived support from parents for physical activity, parent's and girl's perceived neighborhood safety and access to facilities, and family environment.

Expression of two succinyl-CoA synthetases with different nucleotide specificities in mammalian tissues.

For nearly 50 years, succinyl-CoA synthetase in animals was thought to be specific for guanine nucleotides. Recently, we purified and characterized both an ADP-forming succinyl-CoA synthetase from pigeon breast muscle and the GDP-forming enzyme from liver (Johnson, J. D.

Transmission, In Planta Distribution, and Management of Hibiscus latent Fort Pierce virus, a Novel Tobamovirus Isolated from Florida Hibiscus.

Three aspects of the infection process of a new tobamovirus species, Hibiscus latent Fort Pierce virus, recently isolated from hibiscus in Florida, were examined: (i) transmission efficiency of rub-, slash-, and cut-inoculation for two hibiscus cultivars, Pink Versicolor and Brilliant Red; (ii) distribution within infected hibiscus plants; and (iii) treatments to prevent infection during plant propagation and pruning. Rub-, slash-, and cut-inoculation methods were all effective and yielded infection rates of 66, 74, and 70%, respectively, in Pink Versicolor and 50, 56, and 38%, respectively, in Brilliant Red. Analysis of virus distribution in infected plants over time revealed that the virus moved from the place of inoculation to the roots and then toward the bottom (oldest) leaves of the plants.

Posttetanic count revisited: are measurements more reliable using the TOF-Watch accelerographic peripheral nerve stimulator?

Objective: Measurement of profound neuromuscular block using posttetanic count is among the most subjective measurements made in clinical anesthesia. The TOF-Watch accelerographic peripheral nerve stimulator provides objective measurements of neuromuscular block that may improve our ability to quantitate intense blockade.

Methods: The TOF-Watch and Digi Stim III peripheral nerve stimulators were used to monitor onset and early recovery of neuromuscular response induced by rocuronium 0.

Success factors in the long-term sustainability of a telediabetes programme.

We studied a telediabetes programme that had been in operation for 10 years, to identify the factors in its sustainability. We reviewed archive documents, conducted interviews with programme administrators and primary care providers, observed diabetes consultations and surveyed patient satisfaction. Three success factors were identified: the administration took a long-term view of the value of the telemedicine service; telediabetes enabled structured use of staff time and facilities; and service delivery followed national diabetes standards and a well defined cycle of care within a long-term quality improvement programme.

Comparison of Detection Methods for a Novel Tobamovirus Isolated from Florida Hibiscus.

A novel tobamovirus recently was isolated from hibiscus in Florida. Serological and molecular methods, including enzyme-linked immunosorbent assay (ELISA), dot-blot immunoassay (DBIA), tissue-blot immunoassay (TBIA), and immunocapture reverse-transcription poly-merase chain reaction (IC-RT-PCR) were compared to evaluate their usefulness for diagnosis of this virus. Each method was tested with partially purified virus preparations and tissue samples from infected hibiscus and Chenopodium quinoa plants.

Development and implementation of a visual card-sorting technique for assessing food and activity preferences and patterns in African American girls.

Objective: Card-sorting tasks for assessing food and activity preferences and patterns among African American girls were developed. Associations among food preference and intake frequency, activity preference and frequency, and body mass index were examined.

Design: Participants completed newly developed card-sorting tasks assessing food and activity preferences and patterns.

Biological and Molecular Characterization of a Novel Tobamovirus with a Unique Host Range.

Tobamoviruses are among the best characterized and most studied plant viruses. Three subgroups of tobamoviruses correspond to viral genome sequence and host range to include those viruses infecting (i) solanaceous plants, (ii) brassicas, or (iii) cucurbits or legumes. We isolated a virus from Florida landscape plantings of the malvaceous plant hibiscus (Hibiscus rosasinensis) that appears to be a tobamovirus based upon its virion morphology, genome organization, and coat protein sequence.

Effects of modification of the transcription initiation site context on citrus tristeza virus subgenomic RNA synthesis.

Citrus tristeza virus (CTV), a member of the Closteroviridae, has a positive-sense RNA genome of about 20 kb organized into 12 open reading frames (ORFs). The last 10 ORFs are expressed through 3'-coterminal subgenomic RNAs (sgRNAs) regulated in both amounts and timing. Additionally, relatively large amounts of complementary sgRNAs are produced.

Cucumber mosaic virus Diagnosed in Desert Rose in Florida.

Desert rose (Adenium obesum (Forssk) Roem. & Schult.) is a member of the family Apocynaceae and characterized by fleshy leaves and stems and colorful flowers.

Tooth color: effects on judgments of attractiveness and age.

Tooth whitening has become a very popular procedure. Advertisements for whitening products imply that whiter teeth are more attractive than yellower teeth. We tested this idea empirically by manipulating the tooth color of pictures of male and female targets.

Impaired cardiac function and IGF-I response in myocytes from calmodulin-diabetic mice: role of Akt and RhoA.

This study characterized the cardiac contractile function and IGF-I response in a transgenic diabetic mouse model. Mechanical properties were evaluated in cardiac myocytes from OVE26 diabetic and FVB wild-type mice, including peak shortening (PS), time to PS (TPS), time to 90% relengthening (TR(90)) and maximal velocity of shortening/relengthening (+/-dL/dt). Intracellular Ca(2+) was evaluated as Ca(2+)-induced Ca(2+) release [difference in fura 2 fluorescent intensity (Delta FFI)] and fluorescence decay rate (tau).

First Report of Tomato spotted wilt virus in Blackberry Lily in North America.

Blackberry lily (Belamcanda chinensis (L.) DC.) is an herbaceous perennial in the Iridaceae characterized by purple-spotted orange flowers followed by persistent clusters of black fruit.

Key West Nightshade, a New Experimental Host for Plant Viruses.

Key West nightshade (Solanum bahamense) is a perennial solanaceous weed found in the extreme southern portion of Florida. It can be propagated by seed and cuttings and is absent from the noxious weed lists of all U.S.

A preoperative intervention for pain reduction, improved mobility, and self-efficacy.

The Foster Pain Intervention (FPI) is a 24-minute videotape of a nurse showing breathing and movement skills with four postoperative mobility activities to a patient. Its preoperative use incorporates self-efficacy concepts to teach techniques that can improve postoperative pain and mobility. This study compared the effects of the FPI on postoperative pain and mobility and the relationship with self-efficacy in 70 elective hysterectomy patients.

Identification of Pepper mild mottle virus in Commercial Bell Pepper in Florida.

Pepper mild mottle virus (PMMoV) has been identified on pepper in Florida several times since 1997. Initial observations were on ornamental peppers (Capsicum chinense, C. frutescens, and C.

Melatonin suppression by light in euthymic bipolar and unipolar patients.

Background: Previous studies have suggested that bipolar patients are supersensitive to light suppression of melatonin and that this may be a trait marker for genetic vulnerability. The present study was an attempt to replicate and extend this observation. Propranolol hydrochloride effects were compared with light effects because of the documented influence of beta-adrenergic receptors on melatonin production.

Tomato spotted wilt virus-positive steps towards negative success.

Abstract Taxonomy: Tomato spotted wilt virus (TSWV) is the type member of the plant-infecting Tospovirus genus in the family Bunyaviridae, a large group of predominantly vertebrate- and insect-infecting RNA viruses. Physical properties: Virions are 80-120-nm pleomorphic particles with surface projections composed of two viral glycoproteins, G1 and G2 (Fig. 1).