Publications by authors named "Aditi Kuppe"
Article Synopsis
- In cystic fibrosis (CF) patients, increased disulfide crosslinking in mucins leads to thicker mucus, causing dysfunction; researchers developed a new compound, dPGS-SH, to reduce these crosslinks.
- dPGS-SH shows strong mucolytic activity in sputum from CF patients, outperforming the existing treatment NAC in terms of reducing multimer intensity of key mucins and improving mucus properties.
- The new polymer, dPGS-SH, is stable, non-toxic, and effective across various pH levels, suggesting it could be a promising option for enhancing mucus clearance in CF and related lung diseases.
Crohn's disease (CD) is an inflammatory bowel disease that can affect any part of the gastrointestinal tract, frequently involving the terminal ileum. While colonic mucus alterations in CD patients have been described, terminal ileal mucus and its mechanobiological properties have been neglected. Our study is the first of its kind to decipher the viscoelastic and network properties of ileal mucus.
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- Recent studies showed that the triple combination CFTR modulator therapy, elexacaftor/tezacaftor/ivacaftor (ETI), enhances lung function and reduces flare-ups in cystic fibrosis (CF) patients with at least one CFTR allele, but its impact on mucus properties, infection, and inflammation was previously unexplored.
- This study tracked changes in mucus rheology, microbiome, and inflammation markers in CF patients aged 12 and older over the first year of ETI therapy.
- Results indicated that ETI significantly improved mucus properties, increased microbiome diversity, and reduced inflammation markers, suggesting ETI positively affects airway health, but did not fully normalize conditions to levels seen in healthy individuals.
Background: Mucin disulfide cross-links mediate pathologic mucus formation in muco-obstructive lung diseases. MUC-031, a novel thiol-modified carbohydrate compound, cleaves disulfides to cause mucolysis. The aim of this study was to determine the mucolytic and therapeutic effects of MUC-031 in sputum from patients with cystic fibrosis (CF) and mice with muco-obstructive lung disease (βENaC-Tg mice).
View Article and Find Full Text PDFThe nasal epithelium represents the first line of defense against inhaled pathogens, allergens, and irritants and plays a key role in the pathogenesis of a spectrum of acute and chronic airways diseases. Despite age-dependent clinical phenotypes triggered by these noxious stimuli, little is known about how aging affects the structure and function of the airway epithelium that is crucial for lung homeostasis and host defense. The aim of this study was therefore to determine age-related differences in structural and functional properties of primary nasal epithelial cultures from healthy children and non-smoking elderly people.
View Article and Find Full Text PDFAdenine nucleotides represent crucial immunomodulators in the extracellular environment. The ectonucleotidases CD39 and CD73 are responsible for the sequential catabolism of ATP to adenosine via AMP, thus promoting an anti-inflammatory milieu induced by the "adenosine halo". AMPD2 intracellularly mediates AMP deamination to IMP, thereby both enhancing the degradation of inflammatory ATP and reducing the formation of anti-inflammatory adenosine.
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