Publications by authors named "Adithya Shankara Subramanian"
Article Synopsis
- Bacteria utilize various carbohydrates to create a wide array of glycans, with amino sugars like -acetylglucosamine (GlcNAc) being fundamental components of their cell walls and exopolysaccharides.
- By expressing NahK, researchers can bypass the hexosamine pathway and directly introduce an analogue called -azidoacetylglucosamine (GlcNAz) for metabolic labeling, confirmed through fluorescence-assisted gel electrophoresis.
- The study found that GlcNAz was primarily incorporated into peptidoglycan (PGN), with lesser incorporation into other polysaccharides, which provides insights for developing carbohydrate-based probes and metabolic engineering techniques.
Article Synopsis
- Bacteria utilize various carbohydrates to create complex structures called glycans, particularly incorporating amino sugars like -acetylglucosamine (GlcNAc) in their cell walls and exopolysaccharides.
- A method was developed to bypass the bacterial hexosamine pathway by expressing NahK, allowing the introduction of a GlcNAc analogue, -azidoacetylglucosamine (GlcNAz), which was confirmed to produce another compound, UDP-GlcNAz, using gel electrophoresis.
- The study found that GlcNAz was primarily incorporated into peptidoglycan (PGN), while lower levels were seen in poly-β-1,6--acetylglucos
Bacteria require polysaccharides for structure, survival, and virulence. Despite their central role in microbiology, few tools are available to manipulate their production. In , the glycosyltransferase complex PgaCD produces poly--acetylglucosamine (PNAG), an extracellular matrix polysaccharide required for biofilm formation.
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