This work reports an efficient method to detect SARS-CoV-2 antibodies in blood samples based on SERS combined with a machine learning tool. For this purpose, gold nanoparticles directly conjugated with spike protein were used in human blood samples to identify anti-SARS-CoV-2 antibodies. The comprehensive database utilized Raman spectra from all 594 blood serum samples.
View Article and Find Full Text PDFIn this study, we developed a biosensor based on the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles (AuNPs) to detect the widely used herbicide glyphosate in food samples. To do so, either cysteamine or a specific antibody for glyphosate were conjugated to the surface of the nanoparticles. AuNPs were synthesized using the sodium citrate reduction method and had their concentration determined via inductively plasma coupled mass spectrometry.
View Article and Find Full Text PDFThe regulation of tolerable levels of ochratoxin A in food for human and animal consumption has been defined in some countries. To meet these levels, simpler, more efficient, and faster analytical methods are being developed to facilitate the identification of this dangerous contaminant in food. Here, we combined gold nanoparticles (AuNPs) with anti-ochratoxin A (OTA) IgG to detect elementary levels of OTA based on Localized Surface Plasmon Resonance.
View Article and Find Full Text PDFGreen synthesis of metallic nanoparticles has become incredibly popular, mainly by minimizing problems of environmental contamination and by being able to reduce, stabilize and potentially functionalize nanomaterials. Such compounds have possible applications in various areas, e.g.
View Article and Find Full Text PDFAntibody-coated nanoparticles have recently attracted considerable attention, with the focus falling on diagnostics. Nevertheless, controlled antibody bioconjugation remains a challenge. Here, we present two strategies of bioconjugation with the aim of evaluating the best approach for the coupling of antibodies on the surface of nanomaterials in an oriented way.
View Article and Find Full Text PDFOne of the main problems of the decellularization technique is the subjectivity of the final evaluation of its efficacy in individual organs. This problem can result in restricted cell repopulation reproducibility and worse responses to transplant tissues. Our proposal is to analyze the optical profiles produced by hearts during perfusion decellularization, as an additional method for evaluating the decellularization process of each individual organ.
View Article and Find Full Text PDFThis article presents a corrosion resistant, maneuverable, and intrinsically safe fiber Bragg grating (FBG)-based temperature optical sensor. Temperature monitoring is a critical activity for the oil and gas industry. It typically involves acquiring the desired parameters in a hazardous and corrosive environment.
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