Effects of direct-fed microbials supplementation on in vitro and ex vivo ruminal fermentation and nutrient degradability in lactating Holstein dairy cows.

We conducted two experiments to evaluate the effect of direct-fed microbials () on fermentation parameters and nutrient degradability with two different approaches using rumen fluid from lactating Holstein dairy cows. In Exp. 1, three doses of a DFM containing and () at doses of 3.

An artificial intelligence approach of feature engineering and ensemble methods depicts the rumen microbiome contribution to feed efficiency in dairy cows.

Genetic selection has remarkably helped U.S. dairy farms to decrease their carbon footprint by more than doubling milk production per cow over time.

A multi-species direct-fed microbial supplement alters the milk lipidome of dairy cows.

The study evaluated the effects of supplementing a multi-species direct-fed microbial (DFM) on the milk lipidome of lactating dairy cows. Twenty-four multiparous Holstein cows (41 ± 7 d in milk) were used in a randomized complete block design with experimental duration of 91 d. Cows were blocked based on energy-corrected milk yield from a 14-d pretreatment period, and were assigned randomly within each block to the following treatments: (1) control (CON): corn silage-based total mixed ration without DFM; or (2) BOV+: basal diet top-dressed with a DFM containing a mixture of (LA-51), (PF-24), (CH201), and (CH200) at 11.

Effects of Multi-Species Direct-Fed Microbial Products on Ruminal Metatranscriptome and Carboxyl-Metabolome of Beef Steers.

We examined the effects of two direct-fed microbial (DFM) products containing multiple microbial species and their fermentation products on ruminal metatranscriptome and carboxyl-metabolome of beef steers. Nine ruminally-cannulated Holstein steers were assigned to 3 treatments arranged in a 3 × 3 Latin square design with three 21-d periods. Dietary treatments were (1) Control (CON; basal diet without additive), (2) Commence (PROB; basal diet plus 19 g/d of Commence), and (3) RX3 (SYNB; basal diet plus 28 g/d of RX3).

Plasma Carboxyl-Metabolome Is Associated with Average Daily Gain Divergence in Beef Steers.

We applied an untargeted metabolomics technique to analyze the plasma carboxyl-metabolome of beef steers with divergent average daily gain (ADG). Forty-eight newly weaned Angus crossbred beef steers were fed the same total mixed ration ad libitum for 42 days. On day 42, the steers were divided into two groups of lowest (LF: = 8) and highest ADG (HF: = 8), and blood samples were obtained from the two groups for plasma preparation.