Identifying the impact of toxicity on stream macroinvertebrate communities in a multi-stressor context based on national ecological and ecotoxicological monitoring databases.

In situ bioassays are used to measure the harmful effects induced by mixtures of toxic chemicals in watercourses. In France, national-scale biomonitoring data are available including invertebrate surveys and in-field chemical toxicity measures with caged gammarids to assess environmental toxicity of mixtures of chemicals. The main objective of our study is to present a proof-of-concept approach identifying possible links between in-field chemical toxicity, stressors and the ecological status.

Nature-based solutions (NbS): A management paradigm shift in practitioners' perspectives on riverbank soil bioengineering.

Nature-based Solutions (NbS) are promoted as practical and theoretical solutions that simultaneously provide human well-being and biodiversity benefits. One example is soil bioengineering using construction techniques based on living vegetation, and is frequently used for riverbank stabilization, flood protection, and erosion control. Compared with civil engineering, NbS offer many advantages such as cost reduction, limited impact on the environment, and production of ecosystem services.

Interest of a multispecies approach in active biomonitoring: Application in the Meuse watershed.

A biomonitoring approach based on a single model species cannot be representative of the contaminations impacts on the ecosystem overall. As part of the Interreg DIADeM program ("Development of an integrated approach for the diagnosis of the water quality of the River Meuse"), a study was conducted to establish the proof of concept that the use of a multispecies active biomonitoring approach improves diagnostic of aquatic systems. The complementarity of the biomarker responses was tested in four model species belonging to various ecological compartments: the bryophyte Fontinalis antipyretica, the bivalve Dreissena polymorpha, the amphipod Gammarus fossarum and the fish Gasterosteus aculeatus.

High-multiplexed monitoring of protein biomarkers in the sentinel Gammarus fossarum by targeted scout-MRM assay, a new vision for ecotoxicoproteomics.

Ecotoxicoproteomics employs mass spectrometry-based approaches centered on proteins of sentinel organisms to assess for instance, chemical toxicity in fresh water. In this study, we combined proteogenomics experiments and a novel targeted proteomics approach free from retention time scheduling called Scout-MRM. This methodology will enable the measurement of simultaneously changes in the relative abundance of multiple proteins involved in key physiological processes and potentially impacted by contaminants in the freshwater sentinel Gammarus fossarum.

How to quantify the links between bioavailable contamination in watercourses and pressures of anthropogenic land cover, contamination sources and hydromorphology at multiple scales?

Active biomonitoring permits the quantification of biological exposure to chemicals through measurements of bioavailable concentrations in biota and biological markers of toxicity in organisms. It enables respective comparison of the levels of contamination between sites and sampling campaigns. Caged gammarids are recently proposed as relevant probes for measuring bioavailable contamination in freshwater systems.

In Situ Reproductive Bioassay with Caged Gammarus fossarum (Crustacea): Part 2-Evaluating the Relevance of Using a Molt Cycle Temperature-Dependent Model as a Reference to Assess Toxicity in Freshwater Monitoring.

Active biomonitoring approaches are now recognized as relevant for monitoring water contamination and toxicity. Nevertheless, due to the confounding influence of variable and uncontrolled environmental conditions such as temperature, biological markers measured on transplanted individuals to assess water quality are difficult to interpret. The purpose of the present study is to propose a methodology for adapting a laboratory test of chronic sublethal toxicity based on the molting cycle of Gammarus fossarum to in situ assays.

Shotgun proteomics datasets acquired on animals sampled from the wild.

This data article associated with the manuscript "Comparative proteomics in the wild: accounting for intrapopulation variability improves describing proteome response in a Gammarus pulex field population exposed to cadmium" refers to the shotgun proteomics analysis performed on 40 animals sampled from the wild. Proteins were extracted, digested with trypsin, and the resulting peptides were identified by tandem mass spectrometry. Here, we present the list of proteins from males and the list of proteins from females that are differentially detected between the Brameloup and the Pollon populations.

De novo transcriptomes of 14 gammarid individuals for proteogenomic analysis of seven taxonomic groups.

Gammarids are amphipods found worldwide distributed in fresh and marine waters. They play an important role in aquatic ecosystems and are well established sentinel species in ecotoxicology. In this study, we sequenced the transcriptomes of a male individual and a female individual for seven different taxonomic groups belonging to the two genera Gammarus and Echinogammarus: Gammarus fossarum A, G.

Comparative proteomics in the wild: Accounting for intrapopulation variability improves describing proteome response in a Gammarus pulex field population exposed to cadmium.

High-throughput proteomics can be performed on animal sentinels for discovering key molecular biomarkers signing the physiological response and adaptation of organisms. Ecotoxicoproteomics is today amenable by means of proteogenomics to small arthropods such as Gammarids which are well known sentinels of aquatic environments. Here, we analysed two regional Gammarus pulex populations to characterize the potential proteome divergence induced in one site by natural bioavailable mono-metallic contamination (cadmium) compared to a non-contaminated site.

Multisubstance Indicators Based on Caged Gammarus Bioaccumulation Reveal the Influence of Chemical Contamination on Stream Macroinvertebrate Abundances across France.

Most anthropogenic stressors affecting freshwater systems are qualitatively known. However, the quantitative assessment of contaminant exposure and effects to aquatic communities is still difficult, limiting the understanding of consequences on aquatic ecosystem functioning and the implementation of effective management plans. Here, multisubstance indicators based on caged gammarid bioaccumulated contamination data are proposed (for metals and persistent organic pollutants, POPs) to map the bioavailable contamination level of freshwater ecosystems at a large spatial scale.

Evaluation of psychiatric hospital wastewater toxicity: what is its impact on aquatic organisms?

The primary source of pharmaceuticals to the aquatic environment is the discharge of wastewater effluents. Pharmaceuticals are a large and diverse group of compounds. Among them, psychotropic substances are particularly interesting to study due to their specific known mode of action.

Ecotoxico-Proteomics for Aquatic Environmental Monitoring: First in Situ Application of a New Proteomics-Based Multibiomarker Assay Using Caged Amphipods.

As a proof of principle, a selected reaction monitoring (SRM) mass spectrometry-based methodology was applied to the simultaneous quantification of dozens of protein biomarkers in caged amphipods (Gammarus fossarum). We evaluated the suitability of the methodology to assess complex field contaminations through its application in the framework of a regional river monitoring network. Thanks to the high throughput acquisition of biomarker levels in G.

Caged Gammarus as biomonitors identifying thresholds of toxic metal bioavailability that affect gammarid densities at the French national scale.

Bioaccumulated concentrations of toxic elements in biomonitor invertebrate species have already been used to successfully link metal bioavailability and impairments of stream macroinvertebrate communities at the scale of the watershed. However, implementing this empirical comparative approach at a greater spatial scale remains a challenge due to the diversity of biogeographical contexts encompassed by regional and national scales. We showed in previous studies that the use of standard organisms caged permits the use of a common biomonitor over a far greater geographical range, while limiting the influence of confounding factors on levels of bioavailable contamination.

Gammarus fossarum as a sensitive tool to reveal residual toxicity of treated wastewater effluents.

Wastewater treatment plants (WWTPs) are one of the main sources of freshwater pollution eventually resulting in adverse effects in aquatic organisms. Treated effluents can contain many micropollutants at concentrations often below the limit of chemical quantification. On a regulatory basis, WWTP effluents have to be non-toxic to the aquatic environment, wherefore not only chemical abatement but also ecotoxicological evaluation through relevant bioassays is required.

Bioaccumulation of Toxoplasma and Cryptosporidium by the freshwater crustacean Gammarus fossarum: Involvement in biomonitoring surveys and trophic transfer.

The protozoa Toxoplasma gondii and Cryptosporidium parvum are public health priorities because their oocysts can persist in recreational, surface, drinking, river, and sea water sources for a long time. To evaluate the capacity of the freshwater crustacean Gammarus fossarum to accumulate T. gondii and C.

Application of a multidisciplinary and integrative weight-of-evidence approach to a 1-year monitoring survey of the Seine River.

Quality assessment of environments under high anthropogenic pressures such as the Seine Basin, subjected to complex and chronic inputs, can only be based on combined chemical and biological analyses. The present study integrates and summarizes a multidisciplinary dataset acquired throughout a 1-year monitoring survey conducted at three workshop sites along the Seine River (PIREN-Seine program), upstream and downstream of the Paris conurbation, during four seasonal campaigns using a weight-of-evidence approach. Sediment and water column chemical analyses, bioaccumulation levels and biomarker responses in caged gammarids, and laboratory (eco)toxicity bioassays were integrated into four lines of evidence (LOEs).

Linking feeding inhibition with reproductive impairment in Gammarus confirms the ecological relevance of feeding assays in environmental monitoring.

The in situ feeding bioassay in Gammarus fossarum is recognized as a reliable tool for monitoring the toxicity of freshwater contamination. However, whether recorded feeding inhibitions can potentially provoke population-level adverse outcomes remains an open question. In the present study, the authors present an experimental study in G.

Seasonal variability and inter-species comparison of metal bioaccumulation in caged gammarids under urban diffuse contamination gradient: implications for biomonitoring investigations.

Although caging of Gammarus species offers promising lines of inquiry to monitor metal bioavailability in freshwaters, the interspecies responsiveness to metal exposures is still unclear. In addition, abiotic factors inherent to transplantation can hamper the interpretation of field bioaccumulation data. To assess the relevance of using gammarids as biomonitors, we investigated the seasonal influence on metal bioaccumulation in two common species, Gammarus pulex and Gammarus fossarum.