Inactivation of and Genes in BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose -Glycans.

Bright Yellow-2 (BY-2) suspension cells are among the most commonly used plant cell lines for producing biopharmaceutical glycoproteins. Recombinant glycoproteins are usually produced with a mix of high-mannose and complex -glycans. However, -glycan heterogeneity is a concern for the production of therapeutic or vaccine glycoproteins because it can alter protein activity and might lead to batch-to-batch variability.