Publications by authors named "Adele Goldman-Pinkovich"
Article Synopsis
- Arginine balance in lysosomes is crucial for the growth and metabolism of mammalian cells, particularly within macrophages where the protozoan parasite causing leishmaniasis resides.
- When infected, the parasite detects low arginine levels through a surface sensor, activating a response that increases the abundance and activity of an arginine transporter, necessary for its survival.
- Disruption of this response through gene editing demonstrated that while the mutants could grow normally in lab settings, they were significantly less effective at developing and infecting within host macrophages and mice, highlighting the importance of arginine sensing for the parasite's pathogenicity.
Amino acid sensing is an intracellular function that supports nutrient homeostasis, largely through controlled release of amino acids from lysosomal pools. The intracellular pathogen Leishmania resides and proliferates within human macrophage phagolysosomes. Here we describe a new pathway in Leishmania that specifically senses the extracellular levels of arginine, an amino acid that is essential for the parasite.
View Article and Find Full Text PDFThe aim of the present study was to investigate the feasibility of targeting Leishmania transporters via appropriately designed chemical probes. Leishmania donovani, the parasite that causes visceral leishmaniasis, is auxotrophic for arginine and lysine and has specific transporters (LdAAP3 and LdAAP7) to import these nutrients. Probes 1-15 were originated by conjugating cytotoxic quinone fragments (II and III) with amino acids (i.
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