Publications by authors named "Adela Saskova"
Aurora kinase A (AURKA) is an important mitotic kinase involved in the G2/M transition, centrosome maturation and separation, and spindle formation in somatic cells. We used transgenic models that specifically overexpress in mouse oocytes either wild-type (WT-AURKA) or a catalytically inactive (kinase-dead) (KD-AURKA) AURKA to gain new insights regarding the role of AURKA during oocyte maturation. AURKA activation occurs shortly after hCG administration that initiates maturation in vivo.
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- Condensins I and II are crucial for chromosome behavior in both mitosis and meiosis, and this study explores their subunits in mammalian oocytes for the first time.
- Through immunoblotting, it was found that while some condensin subunits like SMC2 did not show significant changes during oocyte maturation, others like CAP-D2 and CAP-D3 exhibited multiple isoforms, suggesting post-translational modifications might be at play.
- The research also showed that CAP-H localizes in the cytoplasm and CAP-H2 in the nucleus, highlighting their distinct roles in oocyte maturation using immunofluorescence staining techniques.
Article Synopsis
- Aurora kinase A (AURKA) is a key enzyme for cell cycle control, particularly in centrosome maturation and spindle assembly, and plays a significant role in the meiotic maturation of mouse oocytes.
- In oocytes, active AURKA localizes to microtubule organizing centers (MTOCs) during the G2 to M phase transition, in contrast to its localization in somatic cells.
- Studies show that AURKA activation at MTOCs is independent of certain signaling pathways, and manipulating AURKA levels affects spindle formation and the progression of meiosis.
CDK1 is a pivotal regulator of resumption of meiosis and meiotic maturation of oocytes. CDC25A/B/C are dual-specificity phosphatases and activate cyclin-dependent kinases (CDKs). Although CDC25C is not essential for either mitotic or meiotic cell cycle regulation, CDC25B is essential for CDK1 activation during resumption of meiosis.
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