Tyrosinase from the pulps of local cultivars of Purification, characterization, immobilization, and application in the batch production of l-3,4-dihydroxyphenylalanine.

Tyrosinase, an enzyme involved in browning reactions in plants/crops exposed to mechanical injury, was isolated from the pulp of some different locally available bananas (, , and ). Tyrosinase from the pulps was extracted, purified, immobilized, and characterized. Thereafter, the potentials of the immobilized tyrosinase in the possible production of l-3,4-dihydroxyphenylalanine (L-DOPA) in an improvised batch reactor was exploited using tyrosine and ascorbate as the substrates.

Comparative modelling, molecular docking and immobilization studies on lipase: evaluation of potentials for fatty acid methyl esters synthesis.

Elucidation of lipase-substrate interactions will guide the proper industrial use and applicability of the enzyme. The aim of this study was to predict the 3 D structure of ZAC3 (ZAC3) lipase, study its interactions with some natural substrates and evaluate the feasibility of fatty acid methyl esters (FAME) production by the immobilized lipase. Protein identification of ZAC3 lipase was carried out using LC-MS/MS.

Bioflocculant produced by Bacillus velezensis and its potential application in brewery wastewater treatment.

This study was designed to evaluate the potential of bioflocculant producing strains isolated from wastewater sludge. According to the Plackett-Burman design, the response surface revealed glucose, magnesium sulfate, and ammonium sulfate as critical media components of the nutritional source, whereas the central composite design affirmed an optimum concentration of the critical nutritional source as 16.0 g/l (glucose), 3.

A heat-resistant intracellular laccase immobilized via cross-linked enzyme aggregate preparation: Characterization, application in bisphenol A removal and phytotoxicity evaluation.

Aureobasidium pullulans laccase was immobilized via cross-linked enzyme aggregate (CLEA) and deployed in bisphenol-A (BPA) removal. The immobilization and BPA removal processes were mathematically modeled. The CLEA-treated BPA was evaluated for phytotoxicity.

Biodegradation of cyanide in cassava wastewater using a novel thermodynamically-stable immobilized rhodanese.

Extracellular rhodanese obtained from was employed in both free and immobilized forms for the biodegradation of cyanide present in cassava processing mill effluent (). Crosslinking with glutaraldehyde (at an optimum concentration of 5% v/v) before entrapment in alginate beads resulted in the highest immobilization yield of 94.5% and reduced enzyme leakage of 1.

Kinetic and thermodynamic investigations of cell-wall degrading enzymes produced by via induction with orange peels: application in lycopene extraction.

The production of cell-wall degrading enzymes () such as cellulase and pectinase by NAC8 through induction using orange peels was investigated for the potential application of these enzymes in the extraction of lycopene from tomato skin, waste, and paste (). The was then immobilized via entrapment in alginate beads for lycopene extraction and the kinetic/thermodynamic properties of the free and immobilized investigated. The optimum production of occurred at pH, temperature, and orange peel concentration of 6.

Strain improvement and statistical optimization as a combined strategy for improving fructosyltransferase production by NAC8.

Strain improvement of a low fructosyltransferase-producing NAC8 (Accession No. KX023301) was carried out using chemical mutagens such as ethidium bromide and ethyl methane sulfonate. The wild-type and mutant strain were distinguished using Random amplified polymorphic DNA PCR and DNA fingerprinting analysis.

Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated NAC8 obtained from soil containing decayed plant matter.

The study investigated the biochemical characteristics and kinetic parameters of laccase from a newly isolated NAC8 obtained from soil containing decay plant litters. This was with a view to identifying the type of laccase and its possible suitability for biotechnological applications. The fungal strain was identified as NAC8 by sequencing of its 5.