The use of computational fluid dynamic models for the optimization of cell seeding processes.

The seeding of a porous scaffold with stem cells is a fundamental step in engineering sizeable tissue constructs that are clinically viable. However, a key problem often encountered is inhomogeneous seeding of the cells particularly when the cells are delivered through the thickness of the scaffold. The objective of this study was to establish the quantitative relationships between the cell seeding efficiency and the initial vacuum pressure in a compact perfusion seeding device that uses the effect of differential pressure induced by vacuum to seed cells on a porous scaffold.