Navigating the Complex Terrain of Methane Synthesis: Multienzyme Control Points and Data-Driven Strategies.

Anaerobic digestion is a crucial process in wastewater treatment, renowned for its sustainable biogas production capabilities and the simultaneous reduction of environmental pollution. However, dysregulation of vital biological processes and pathways can lead to reduced efficiency and suboptimal biogas output, which can be seen through low counts per million of sequences related to three critical control points for methane synthesis. Namely, tetrahydromethanopterin S-methyltransferase (MTR), methyl-coenzyme reductase M (MCR), and CoB/CoM heterodisulfide oxidoreductase (HDR) are the last reactions that must occur.

A unique xylose reductase from Thermomyces lanuginosus: Effect of lignocellulosic substrates and inhibitors and applicability in lignocellulosic bioconversion.

In this study, the xylose reductase gene (XRTL) from Thermomyces lanuginosus SSBP was expressed in Pichia pastoris GS115 and Saccharomyces cerevisiae Y294. The purified 39.2 kDa monomeric enzyme was optimally active at pH 6.

Comparative biocontrol ability of chitinases from bacteria and recombinant chitinases from the thermophilic fungus Thermomyces lanuginosus.

Microbial chitinases (EC 3.2.1.

Concomitant production of chitosan and lipids from a newly isolated Mucor circinelloides ZSKP for biodiesel production.

A newly-isolated oleaginous fungus Mucor circinelloides ZSKP concurrently yielded 21.4% lipids and 11.2% chitosan per gram of biomass.

Thermo-acid-stable phytase-mediated enhancement of bioethanol production using Colocasia esculenta.

Phytase production by the thermophilic mould Thermomyces lanuginosus SSBP was enhanced 8.56-fold in submerged fermentation, which was further improved in fed-batch cultivations. The protein was purified to homogeneity using ammonium sulphate precipitation, Resource Q anion exchange and Superdex gel-filtration chromatography, with an overall purification of 24.

Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS.

Optimization of process parameters for phytase production by Enterobacter sp. ACSS led to a 4.6-fold improvement in submerged fermentation, which was enhanced further in fed-batch fermentation.