Publications by authors named "Adara Bochanis"
Article Synopsis
- - Antisense oligonucleotides (ASOs) are single-stranded nucleic acids that specifically target RNA and have been approved by the FDA for various diseases, utilizing three main mechanisms of action: mRNA degradation, exon skipping, and mRNA function inhibition.
- - ASOs are structurally modified for stability and resistance to degradation, and examples include drugs like inotersen for hereditary transthyretin amyloidosis and nusinersen for spinal muscular atrophy.
- - The design of ASOs is largely based on mRNA sequence knowledge, making the development process quicker compared to traditional protein-targeted drugs, which enhances their potential for therapeutic applications.
Objectives: The final stage of liver development is the production of hepatocytes and cholangiocytes (biliary epithelial cells) from bipotent hepatic progenitor cells. We used HepaRG cells, which are bipotent and able to differentiate into both hepatocytes and cholangiocytes, as a model to study the action of a novel lncRNA (lnc-RHL) and its role in the regulation of bipotency leading to hepatocytes and cholangiocytes.
Materials And Methods: Differentiation of HepaRG cells was assessed by marker expression and morphology which revealed their ability to differentiate into hepatocytes and cholangiocytes (modelling the behaviour of hepatoblasts in vivo).