PHRF1 Promotes Cell Invasion by Modulating SOX4 Expression in Colorectal Cancer HCT116-p53 Cells.

Background/aim: PHD and RING finger domain-containing protein 1 (PHRF1) ubiquitinates TGIP (TG-interacting protein) and redistributes cPML (cytoplasmic variant of PML) to the cytoplasm to enhance TGF-β signaling by. It is unclear whether PHRF1 affects invasion and survival when both mutations of the activated oncogene Kras and inactivation of the tumor suppressor p53 are present.

Materials And Methods: We knockout PHRF1 expression using Crispr-Cas9 editing in HCT116-p53 (KrasG13D/p53) cells and analyzed the expression profile in HCT116-p53PHRF1 cells.