Publications by authors named "Adao R da Silva"
The screening of rare blood donors in a highly admixed population: A new approach for Holley and Diego genotyping and impact of genomic and self-reported ancestry.
Amanda A Muniz Adão R da Silva Isabela A Ferraz Marina L Martins Mariana M Godin Maria Clara Fernandes da Silva Malta
Transfus Med
April 2020
Objectives: The present study aimed to develop strategies for genotyping DO*HY (Dombrock system) and DI*A/DI*B (Diego system) alleles and to evaluate the impact of genomic and self-declared ancestry on rare donor screening in admixed populations.
Background: The antigens Hy and Di demonstrate clinical importance. The lack of antisera for the serological evaluation of these antigens makes it necessary to develop molecular methods.
Article Synopsis
- The Duffy blood group system is important for transfusion medicine, immunology, and malaria research, but current genotyping methods have limitations and lack widespread data in some areas.
- A new real-time PCR-based method for genotyping Duffy alleles was developed and tested on blood donors and transfusion-dependent patients in Minas Gerais, Brazil, revealing no key differences in allele frequencies between groups.
- The study found that while the Fy(a-b+) phenotype was common, the Fy(a-b-) phenotype, linked to African descent, was less frequent among self-identified black individuals, indicating a potential link to malaria susceptibility and blood antigen risks in the region.