[Metabolic immunocorrection treatment of experimental widespread purulent peritonitis].

The effect of citoflavin and neoton preparations on the migration activity of neutrophil granulocytes under action of mitogen-induced immunocompetent blood cells, Peyer's patches, spleen and inguinal lymph was studied on a group of 55 male chinchilla rabbits with experimental model of widespread purulent peritonitis. It is established that the regulating action of immunocompetent cells on the migration of neutrophil leukocytes under the conditions of widespread purulent peritonitis is stable and widespread process, which is observed within 5 days of the postoperative period. The use of citoflavin and neoton during the postoperative period produces correction of the activity of immunocompetent cells, changing their properties in regulation of the migratory activity of neutrophil granulocytes.

[Clinical and laboratory assessment of interactions between poly- and mononuclear phagocytes in inflammatory diseases of the skin].

Interactions between polymorphonuclear leukocytes (PMNL) and monocytes are changed in inflammatory diseases of the skin; these changes manifest by enhanced stimulation of PMNL migration under the effect of monocytes in psoriasis and by depression of this migration in eczema. In psoriasis HLA-DR-antigens and endogenic monocyte prostaglandins participate in this modulation of cooperative interactions between phagocytic cells, whereas in eczema PMNL superoxide anion takes part in this process. These data may be useful in the diagnosis of inflammatory diseases of the skin and disclose new aspects in the pathogenesis of psoriasis and eczema connected with phagocytic system cells.

[Method of the assessment of neutrophilic leukocyte and monocyte interactions in a mixed cell culture].

The author offers a test system for studies of the interactions of agglutinating polymorphonuclear cells and neutrophilic leukocytes of human blood in a mixed cell culture. This method has demonstrated a modulation of the structural and functional status of the interacting cells. The counts of polymorphonuclear cells, expressing HLA-DR antigens, and of neutrophilic leukocytes with receptors to Fc fragment of IgG grow in the course of cell cultivation, and a number of cytokines, interleukin-1 and factors of chemotaxis of polymorphonuclear cells and neutrophilic leukocytes, are produced.

[The effect of toluene and ammonia on the invasive process and immunological indices in experimental trichinelliasis in mice].

Rap mice were exposed to toluene (T) inhalation for 10 days before invasion with 20 Trichinella spiralis larvae per g body weight (moderate infection). This resulted in diminished number of intestinal parasites in the presence of greater number of mast cells in the peritoneal exudate, higher IgE production, enhanced cell adhesion to trichinella larvae and of migration of splenic lymphocytes. Simultaneous inhalation of T and ammonia diminished the immune stimulating effect of the former.

[Development and a clinical test of a method of assessing the interactions between polymorphonuclear leukocytes and monocytes in human blood].

A method for assessment of interactions between blood polymorphonuclear leukocytes and monocytes is described, demonstrating the monocyte ability to stimulate polymorphonuclear leukocyte migration in capillary leukocyte migration test. Clinical trials of the method have shown enhanced monocyte stimulation of the migration of polymorphonuclear leukocytes in psoriasis patients during exacerbations of the disease. The method may be used for assessment of the immune status to detect disorders at the level of intercellular relations.

[Immunologic parameters of blood after extensive intestinal resection].

A complex clinico-immunological examination of patients subjected to extensive resections of different portions of the intestine was performed. It was established that participation of the immune system in regulation of homeostasis in patients under examination was characterized by lympho- and monocytopenia, lower amount of T-lymphocytes at the expense of low avidity fractions with a simultaneous increase of high avidity fractions of these cells.

[The functional characteristics of the interaction of lymphokine-producing lymphocytes with granulocytes of the peripheral blood and skin in dermatosis patients].

PHA-induced mononuclear production of lymphokines altering leukocyte migration was studied in patients with psoriasis, mycosis, and scabies. The findings evidence that T-cells synthesize leukocyte migration inhibition factor (LMIF) in mycosis and scabies, whereas in psoriasis they produce both LMIF and leukocyte migration stimulation factor (LMSF). Blood leukocytes of mycosis and scabies patients were more sensitive to LMIF than the cells of psoriasis patients.

[A method of determining interleukin-1 in a culture of lymphocytes and human blood monocytes activated by a pulsed method].

Human peripheral blood monocyte monolayer was treated with lipopolysaccharide by the pulsed method. As a result of such activation the cells could produce interleukin-1, that enhanced the proliferation of lymphocytes added to the activated monocyte culture together with suboptimal dose of phytohemagglutinin.

[The functional properties of interleukins in psoriasis].

IL-1 and IL-2 interleukins have been studied in patients with diffuse psoriasis en papules et plaques in the stationary stage. The results evidence increased production of IL-1 and its reduced reception by the immunocompetent cells. The lymphocytes have been characterized by a reduced IL-2 synthesis and its lower binding with IL-2-sensitive cells.

[Replacement of the helper function of T cells by an RNA-containing antigen-specific lysis factor].

The lymph node viable cells suspension of immunized mice was centrifugated. The supernatant was chromatographed in Sephadex G-200, and fractions were deproteinized. The deproteinized third fraction (Mol wt 30000) stimulated specifically the plaque-forming cells of intact mice immunized by SRBC.

[Immunochemical characteristics of a factor causing antigen-specific lysis of thymus-dependent lymphocytes].

A factor capable of lysing in vitro, in the presence of a specific antigen, the cells of the lymph nodes and the thymus of intact mice was revealed in the supernatant obtained after the centrifugation of a suspension of viable cells of the lymph nodes and the thymus of the immunized mice. It was found by immunochemical methods that this factor had a mol. wt.

[Determining hypersensitivity to antigens by computing cells migrating from capillary tubes].

The test technique of inhibition of leukocyte and macrophage migration from the capillaries in the presence of a specific antigen is described. The method is highly sensitive and permitted to use a small amount of cells and many (up to 600 and more) capillaries. Sodium azide, phitohemagglutinin and incubation at 4 degrees C inhibited the cell migration from the capillaries.

[Study of bacterial allergy in acute appendicitis by the method of leukocyte migration inhibition in vitro].

Under study was the effect of various bacterial allergens on the migration of blood leucocytes from capillaries in vitro in 23 patients with acute appendicitis and in 18 healthy individuals. Bacterial allergens wound suppress leucocytes migration in all patients. Most frequently, sensibilization to allergens of hemolytic staphylococcus and hemolytic streptococcus was found.

[Antigen-specific lysis in vitro of lymph node cells of intact mice injected with RNA from viable lymph node cells of immunized animals].

Supernatant fluid obtained after centrifugation of the suspension of viable lymph node cells of immunized animals proved to induce in vivo in the lymph node cells of intact mice sensitivity to lysis with a specific antigen in vitro. This property was possessed after chromatography of the supernatant fluid on Sephadex G-200 by the 3rd fraction (MW about 30000 dalton). DNA-ase, trypsin or deproteinization failed to influence whereas RNA-ase inactivated this fraction in respect to the inducing properties.