[Pharmacokinetic design for the preclinical phase. Application to the study of an anthracenone].

T-514 is a toxic substance of Karwinskia humboldtiana which has been described as a possible anticancer agent. An animal model (New Zeland white rabbit) was selected for pharmacokinetic studies that would allow the performance of surgical techniques to catheterize central vessels in order to obtain blood samples at short time intervals. A nonlinear regression analysis method was used to fit the plasmatic concentration data to a multiexponential mathematical function; a computer program based on Marquant's iterating algorithm was used to fit the experimental curves.