Publications by authors named "Adam Kubrak"
Raman microscopy reveals how cell inflammation activates glucose and lipid metabolism.
Aleksandra Borek-Dorosz Anna Pieczara Jagoda Orleanska Krzysztof Brzozowski William Tipping Adam Kubrak
Biochim Biophys Acta Mol Cell Res
January 2024
Article Synopsis
- - The study investigates how glucose metabolism in endothelial cells (ECs) changes during inflammation using human microvascular endothelial cells (HMEC-1) and Raman imaging techniques.
- - By treating ECs with deuterated glucose and inflammatory cytokine TNF-α, researchers observed significant metabolic changes, including increased glucose uptake and lipid production, particularly of unsaturated lipids.
- - Findings suggest that enhanced mitochondrial activity and lipid metabolism could play a role in cardiovascular disease, with Raman spectroscopy being a valuable tool for identifying metabolic changes related to endothelial dysfunction.
Nuclear magnetic resonance (NMR) relaxometry is an analytical method that provides information about molecular environments, even for NMR "silent" molecules (spin-0), by analyzing the properties of NMR signals versus the magnitude of the longitudinal field. Conventionally, this technique is performed at fields much higher than Earth's magnetic field, but our work focuses on NMR relaxometry at zero and ultra-low magnetic fields (ZULFs). Operating under such conditions allows us to investigate slow (bio)chemical processes occurring on a timescale from milliseconds to seconds, which coincide with spin evolution.
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