Effects of Glutathione on Hydrolytic Enzyme Activity in the Mouse Hepatocytes.

In this study, the effect of glutathione (GSH) on the activity of hydrolytic enzymes of lysosomal, microsomal, and cytosolic fractions was studied in the mouse hepatocytes. The experiments involved 30 Swiss male mice, divided into the experimental and control groups of 15 mice each. The former group received injections of 12 μL/g of GSH solution at a dose of 100 μg/g body weight, whereas the latter received 12 μL/g of physiological saline, all given intraperitoneally daily for 7 days.

Activities of Lysosomal Enzymes in Alloxan-Induced Diabetes in the Mouse.

The study investigated a panel of lysosomal enzymes in the liver and kidney tissues in alloxan-induced diabetes in the mouse. The mice were divided into six experimental groups receiving 10% alloxan at a dose of 50 and 75 mg/kg over a period of four, eight, and twelve days; each group was compared with controls receiving 0.9% NaCl.

The effect of melatonin on glutathione and glutathione transferase and glutathione peroxidase activities in the mouse liver and kidney in vivo.

Objectives: The changes in reduced glutathione (GSH), activity of glutathione transferase (GST) and glutathione peroxidase (GSPx) after administration of exogenous melatonin (N-acetyl-5-methoxy-tryptamine) at a dose 20 mg/kg b.w. were investigated for five and ten days in the liver and kidney of male and female mice.

The influence of scopolamine and extreme temperatures on the lipid level in some mouse organs.

Objectives: The effect of scopolamine administration at dose 0,5 g/kg b.w. and high (+40 degrees C) and low (+4 degrees C) temperature on the level of triacyloglycerides, total lipids and cholesterol in the mouse liver, kidney and muscle of males and females.

Effect of ghrelin on activities of some lysosomal hydrolases in rabbits.

Objectives: Observing the changes of activity of some lysosomal enzymes in blood serum of female rabbits subjected to injection of 10 microg of ghrelin/kg of body weight.

Methods: In the blood serum the activity of cathepsins D and L, alanine aminopeptidase, acid phosphatase, lysosomal lipase and lysosomal esterase was determined.

Results: As a result of ghrelin injection the activity of all the enzymes examined in blood serum increased markedly.

Insulin-induced changes of proteolytic activity of the lysosomal enzymes.

Objectives: Changes in the activity of alanine aminopeptidase, leucine aminopeptidase and cathepsins D and L in the liver and kidney of male and female of mice, injected with 0.4 IU/kg b.w.

Effect of hydrocortisone on the activity of some lysosomal enzymes in mice.

Objectives: Changes in the activity of cathepsin D and L, alanine aminopeptidase, leucine aminopeptidase, N-acetyl-beta-glucosaminidase, lysosomal arylesterase and lysosomal lipase in the liver and kidney of unselected and selected mice, subjected to 7.5 mg/kg b.w.