High-Throughput Determination of Enantiopurity by Microplate Circular Dichroism.

Methods for the rapid determination of enantiomeric excess (ee) in asymmetric synthetic methodology development are increasingly in demand as high-throughput experimentation protocols in academia and industry are adopted. Optical approaches have been reported, many of which rely on the use of chemical derivatization or molecular assemblies, resulting in UV/vis, fluorescence, or circular dichroism (CD) signals that report the ee values. While UV/vis and fluorescence approaches benefit from readily available 96- and 384-well plate readers, until recently, no CD plate readers existed.

Inpatient illness severity surveys provide essential data for planning capacity and managing patient flow in the acute hospital setting.

Purpose: We aimed to determine if Modified Early Warning Score could be used as a surrogate for the Association of United Kingdom University Hospitals dependency scoring in improving patient flow into higher areas of care. In particular, focus was to be placed on the impact of Critical Care expansion on the size of the populations of patients being managed outside of Critical Care with an Association of United Kingdom University Hospitals requirement of Level 2.

Materials And Methods: We conducted snapshot assessments of illness severity using Modified Early Warning Score and Association of United Kingdom University Hospitals dependency scores on all inpatients in a large, rural acute hospital during two five-day periods.

Neural respiratory drive measurement for COPD assessment and monitoring.

Currently there is an unmet need for more objective assessments that could determine COPD severity. Ideally such objective assessments could also anticipate COPD exacerbations in order to decrease the need for repeated hospital admissions. In this review we outline how patients' neural respiratory drive (NRD) may be determined using the electromyography of the diaphragm as an objective measurement of COPD severity.

Two dimensional nanoarrays of individual protein molecules.

Protein molecules on solid surfaces are essential to a number of applications, such as biosensors, biomaterials, and drug delivery. In most approaches for protein immobilization, inter-molecular distances on the solid surface are not controlled and this may lead to aggregation and crowding. Here, a simple approach to immobilize individual protein molecules in a well-ordered 2D array is shown, using nanopatterns obtained from a polystyrene-block-poly(2-hydroxyethyl methacrylate) (PS-b-PHEMA) diblock copolymer thin film.