Hydraulic segmentation explains differences in loss of branch conductance caused by fire.

The hydraulic death hypothesis suggests that fires kill trees by damaging the plant's hydraulic continuum in addition to stem cambium. A corollary to this hypothesis is that plants that survive fires possess 'pyrohydraulic' traits that prevent heat-induced embolism formation in the xylem and aid post-fire survival. We examine whether hydraulic segmentation within stem xylem may act as such a trait.

Consistent responses to moisture stress despite diverse growth forms within mountain fynbos communities.

Understanding climate change impacts on the Cape Floristic Region requires improved knowledge of plant physiological responses to the environment. Studies examining physiological responses of mountain fynbos have consisted of campaign-based measurements, capturing snapshots of plant water relations and photosynthesis. We examine conclusions drawn from prior studies by tracking in situ physiological responses of three species, representing dominant growth forms (proteoid, ericoid, restioid), over 2 years using miniature continuous sap flow technology, long-term observations of leaf/culm water potential and gas exchange, and xylem vulnerability to embolism.

Does a trade-off between growth plasticity and resource conservatism mediate post-fire shrubland responses to rainfall seasonality?

Growth plasticity may allow fire-prone species to maximize their recovery rates during temporary, sporadic periods of rainfall availability in the post-fire environment. However, moisture-driven growth plasticity could be maladaptive in nutrient-limited environments that require tighter control of growth and resource use. We investigated whether a trade-off between plasticity and conservatism mediates growth responses to altered rainfall seasonality in neighbouring shrubland communities that occupy different soils.

Seasonal variation in tap water δH and δO isotopes reveals two tap water worlds.

Stable isotope ratios of hydrogen and oxygen (δH and δO) in tap water provide important insights into the way that people interact with and manage the hydrological cycle. Understanding how these interactions vary through space and time allows for the management of these resources to be improved, and for isotope data to be useful in other disciplines. The seasonal variation of δH and δO in tap water within South Africa was assessed to identify municipalities that are supplied by seasonally invariant sources that have long residence periods, such as groundwater, and those supplied by sources that vary seasonally in a manner consistent with evapoconcentration, such as surface water-the proposed two tap water "worlds".

Maintenance of active chromatin states by HMGN2 is required for stem cell identity in a pluripotent stem cell model.

Background: Members of the HMGN protein family modulate chromatin structure and influence epigenetic modifications. HMGN1 and HMGN2 are highly expressed during early development and in the neural stem/progenitor cells of the developing and adult brain. Here, we investigate whether HMGN proteins contribute to the chromatin plasticity and epigenetic regulation that is essential for maintaining pluripotency in stem cells.

Ramification has little impact on shoot hydraulic efficiency in the sexually dimorphic genus (Proteaceae).

Despite the diversity of branching architectures in plants, the impact of this morphological variation on hydraulic efficiency has been poorly studied. Branch junctions are commonly thought to be points of high hydraulic resistance, but adjustments in leaf area or xylem conduit abundance or dimensions could compensate for the additional hydraulic resistance of nodal junctions at the level of the entire shoot. Here we used the sexually dimorphic genus (Proteaceae) to test whether variation in branch ramification impacts shoot hydraulic efficiency.

Isotopic assessment of marine food consumption by natural-foraging chacma baboons on the Cape Peninsula, South Africa.

Objectives: Stable isotope analysis has been used to investigate consumption of marine resources in a variety of terrestrial mammals, including humans, but not yet in extant nonhuman primates. We sought to test the efficacy of stable isotope analysis as a tool for such studies by comparing isotope- and observation-based estimates of marine food consumption by a troop of noncommensal, free-ranging chacma baboons.

Materials And Methods: We determined δ C and δ N values of baboon hair (n = 9) and fecal samples (n = 144), and principal food items (n = 362).

The Nebulous Ecology of Native Invasions.

In the Anthropocene, alien species are no longer the only category of biological organism establishing and rapidly spreading beyond historical boundaries. We review evidence showing that invasions by native species are a global phenomenon and present case studies from Southern Africa, and elsewhere, that reveal how climate-mediated expansions of native plants into adjacent communities can emulate the functional and structural changes associated with invasions by alien plant species. We conclude that integrating native invasions into ecological practice and theory will improve mechanistic models and better inform policy and adaptive ecological management in the 21st century.

Woodland expansion in South African grassy biomes based on satellite observations (1990-2013): general patterns and potential drivers.

Increases in woody plant cover in savanna grassland environments have been reported on globally for over 50 years and are generally perceived as a threat to rangeland productivity and biodiversity. Despite this, few attempts have been made to estimate the extent of woodland increase at a national scale, principally due to technical constraints such as availability of appropriate remote sensing products. In this study, we aimed to measure the extent to which woodlands have replaced grasslands in South Africa's grassy biomes.

Wind drives nocturnal, but not diurnal, transpiration in Leucospermum conocarpodendron trees: implications for stilling on the Cape Peninsula.

Surface winds have declined in many regions of the world over the past few decades. These trends are referred to as global stilling and have recently been observed in the Western Cape Province of South Africa. The potential consequences of such changes on ecosystem function and productivity are a particular concern for the highly diverse and endemic local flora, largely associated with the fynbos biome.

Experimental evidence for heat plume-induced cavitation and xylem deformation as a mechanism of rapid post-fire tree mortality.

Recent work suggests that hydraulic mechanisms, rather than cambium necrosis, may account for rapid post-fire tree mortality. We experimentally tested for xylem cavitation, as a result of exposure to high-vapour-deficit (D) heat plumes, and permanent xylem deformation, as a result of thermal softening of lignin, in two tree species differing in fire tolerance. We measured percentage loss of conductance (PLC) in distal branches that had been exposed to high-D heat plumes or immersed in hot water baths (high temperature, but not D).

Predicting plant vulnerability to drought in biodiverse regions using functional traits.

Attempts to understand mechanisms underlying plant mortality during drought have led to the emergence of a hydraulic framework describing distinct hydraulic strategies among coexisting species. This framework distinguishes species that rapidly decrease stomatal conductance (gs), thereby maintaining high water potential (Px; isohydric), from those species that maintain relatively high gs at low Px, thereby maintaining carbon assimilation, albeit at the cost of loss of hydraulic conductivity (anisohydric). This framework is yet to be tested in biodiverse communities, potentially due to a lack of standardized reference values upon which hydraulic strategies can be defined.

Drought and resprouting plants.

Many species have the ability to resprout vegetatively after a substantial loss of biomass induced by environmental stress, including drought. Many of the regions characterised by ecosystems where resprouting is common are projected to experience more frequent and intense drought during the 21st Century. However, in assessments of ecosystem response to drought disturbance there has been scant consideration of the resilience and post-drought recovery of resprouting species.

Can stable isotopes and radiocarbon dating provide a forensic solution for curbing illegal harvesting of threatened cycads?

Cycads in South Africa are facing an extinction crisis due to the illegal extraction of plants from the wild. Proving wild origin of suspect ex situ cycads to the satisfaction of a court of law is difficult, limiting law enforcement efforts. We investigated the feasibility of using multiple stable isotopes to identify specimens removed from the wild.

Chromatin looping defines expression of TAL1, its flanking genes, and regulation in T-ALL.

TAL1 is an important regulator of hematopoiesis and its expression is tightly controlled despite complexities in its genomic organization. It is frequently misregulated in T-cell acute lymphoblastic leukemia (T-ALL), often due to deletions between TAL1 and the neighboring STIL gene. To better understand the events that lead to TAL1 expression in hematopoiesis and in T-ALL, we studied looping interactions at the TAL1 locus.

External heat-pulse method allows comparative sapflow measurements in diverse functional types in a Mediterranean-type shrubland in South Africa.

There has been limited application of sapflow technology to small-stemmed species and across co-existing functional types, restricting its use in diverse floras such as the Mediterranean-type shrubland in South Africa. Our main objective was to test whether sapflow may provide an alternative to traditional gas-exchange measurements, which would permit comparative evaluation of transpiration at a previously unattained temporal resolution. We tested miniature external heat ratio method (HRM) sapflow gauges on three co-occurring functional types with contrasting stem or culm anatomies and examined the relationship between sapflow and shoot- and leaf-level water loss in both a controlled and field environment.

Unique reporter-based sensor platforms to monitor signalling in cells.

Introduction: In recent years much progress has been made in the development of tools for systems biology to study the levels of mRNA and protein, and their interactions within cells. However, few multiplexed methodologies are available to study cell signalling directly at the transcription factor level.

Methods: Here we describe a sensitive, plasmid-based RNA reporter methodology to study transcription factor activation in mammalian cells, and apply this technology to profiling 60 transcription factors in parallel.

The inducible tissue-specific expression of the human IL-3/GM-CSF locus is controlled by a complex array of developmentally regulated enhancers.

The closely linked human IL-3 and GM-CSF genes are tightly regulated and are expressed in activated T cells and mast cells. In this study, we used transgenic mice to study the developmental regulation of this locus and to identify DNA elements required for its correct activity in vivo. Because these two genes are separated by a CTCF-dependent insulator, and the GM-CSF gene is regulated primarily by its own upstream enhancer, the main objective in this study was to identify regions of the locus required for correct IL-3 gene expression.

USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator.

The nuclear genomes of vertebrates show a highly organized program of DNA replication where GC-rich isochores are replicated early in S-phase, while AT-rich isochores are late replicating. GC-rich regions are gene dense and are enriched for active transcription, suggesting a connection between gene regulation and replication timing. Insulator elements can organize independent domains of gene transcription and are suitable candidates for being key regulators of replication timing.

Chromatin insulator elements: establishing barriers to set heterochromatin boundaries.

Epigenomic profiling has revealed that substantial portions of genomes in higher eukaryotes are organized into extensive domains of transcriptionally repressive chromatin. The boundaries of repressive chromatin domains can be fixed by DNA elements known as barrier insulators, to both shield neighboring gene expression and to maintain the integrity of chromosomal silencing. Here, we examine the current progress in identifying vertebrate barrier elements and their binding factors.

Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.

Genomic maps of chromatin modifications have provided evidence for the partitioning of genomes into domains of distinct chromatin states, which assist coordinated gene regulation. The maintenance of chromatin domain integrity can require the setting of boundaries. The HS4 insulator element marks the 3' boundary of a heterochromatin region located upstream of the chicken β-globin gene cluster.