Publications by authors named "Adam F Prasanphanich"
Purpose: To evaluate the feasibility of catheter-directed intra-arterial stem cell delivery of human mesenchymal stem cells (MSCs) to the small bowel in a porcine model.
Materials And Methods: The cranial mesenteric artery of 6 Yucatan minipigs was selectively catheterized under fluoroscopic guidance following cut-down and carotid artery access. A proximal jejunal branch artery was selectively catheterized for directed delivery of embolic microspheres (100-300 μm) or MSCs (0.
The side population (SP) assay, a technique used in cancer and stem cell research, assesses the activity of ABC transporters on Hoechst staining in the presence and absence of transporter inhibition, identifying SP and non-SP cell (NSP) subpopulations by differential staining intensity. The interpretation of the assay is complicated because the transporter-mediated mechanisms fail to account for cell-to-cell variability within a population or adequately control the direct role of transporter activity on staining intensity. We hypothesized that differences in dye kinetics at the single-cell level, such as ABCG2 transporter-mediated efflux and DNA binding, are responsible for the differential cell staining that demarcates SP/NSP identity.
View Article and Find Full Text PDFPhenotype reprogramming during transforming growth factor β (TGFβ)-induced epithelial-mesenchymal transition (EMT) is an extensive and dynamic process, orchestrated by the integration of biological signaling across multiple time scales. As part of the numerous transcriptional changes necessary for EMT, TGFβ-initiated Smad3 signaling results in remodeling of the redox environment and decreased nucleophilic tone. Because Smad3 itself is susceptible to attenuated activity through antioxidants, the possibility of a positive feedback loop exists, albeit the time scales on which these mechanisms operate are quite different.
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- The study focuses on a substance derived from breast cancer cells that targets the gastrin-releasing peptide receptor (GRPR) using a radionuclide for imaging and therapy.
- The imaging probe combines a specific peptide with a radionuclide and shows promise in effectively visualizing GRPR-positive tumors in laboratory mice through advanced imaging techniques.
- Results indicate that this imaging agent successfully accumulates in tumors, suggesting its potential as a diagnostic tool and a foundation for developing improved cancer-targeting therapies.
Radiolabeled peptides hold promise as diagnostic/therapeutic targeting vectors for specific human cancers. We report the design and development of a targeting vector, [(64)Cu-NOTA-8-Aoc-BBN(7-14)NH(2)] (NOTA = 1,4,7-triazacyclononane-1,4,7-triacetic acid, 8-Aoc = 8-aminooctanoic acid, and BBN = bombesin), having very high selectivity and affinity for the gastrin-releasing peptide receptor (GRPr). GRPrs are expressed on a variety of human cancers, including breast, lung, pancreatic, and prostate, making this a viable approach toward site-directed localization or therapy of these human diseases.
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- A study investigates bombesin (BBN) as a delivery method for diagnostic probes like 99mTc radiometal to target cancers that over-express gastrin-releasing peptide receptors (GRPr).
- Various amino acid and aliphatic compounds were tested to determine how they affect the targeting agent's ability to bind to receptors and its distribution in tissues.
- The study reveals that some new 99mTc-BBN conjugates show significant effectiveness in targeting prostate cancer tumors in rodent models, suggesting potential for improved cancer imaging techniques.