Association of low testosterone with changes in non-cardiovascular biomarkers in adult men.

Testosterone has effects on many organs and systems. The purpose of this study was to test the hypothesis that low testosterone is associated with changes in various non-cardiovascular biomarkers in men older than 40 who were tested for possible hypogonadism. We extracted data from 9939 outpatient men who were over 40 years old (median age 56) and who also had concurrent laboratory measurements of total testosterone and one or more biomarkers of interest: estradiol, uric acid, prostate-specific antigen (PSA), sex-hormone binding globulin (SHBG), luteinizing hormone, creatinine, bone alkaline phosphatase (BAP), creatine kinase, hemoglobin A1c, and 25-hydroxy-vitamin D, and body mass index (BMI).

In-process microbial testing: statistical properties of a rapid alternative to compendial enumeration methods.

Unlabelled: In-process tests are used between manufacturing steps to avoid the cost of further processing material that is apt to fail its final tests. Rapid microbiological methods that return simple negative or positive results are attractive in this context because they are faster than the compendial methods used at product release. However, using a single such test will not reliably detect barely unacceptable material (sensitivity) without generating an undesirable number of false rejections (poor specificity).

Lytic effect of di- or tricarboxylic acids plus sodium dodecyl sulfate against Pseudomonas aeruginosa.

Pseudomonas aeruginosa was rapidly lysed and killed when treated with several multiple-carboxylic acids at 100 micrograms/ml, followed by exposure to sodium dodecyl sulfate at 5 mg/ml.

Isolation of atypical lipopolysaccharides from purified cell walls of Pseudomonas cepacia.

Wall fragments were prepared from two strains of Pseudomonas cepacia and from P. aeruginosa, and their contents of readily extractable lipid, pronase-digestible protein and lipopolysaccharide were measured. Lipopolysaccharide extracted from P.

Fourteen-year survival of Pseudomonas cepacia in a salts solution preserved with benzalkonium chloride.

A strain of Pseudomonas cepacia that survived for 14 years (1963 to 1977) as a contaminant in an inorganic salt solution which contained commercial 0.05% benzalkonium chloride (CBC) as an antimicrobial preservative, was compared to a recent clinical isolate of P. cepacia.

Resistance of spheroplasts and whole cells of Pseudomonas cepacia to polymyxin B.

Sucrose-lysozyme spheroplasts were prepared from two strains of Pseudomonas cepacia and tested for susceptibility to polymyxin B and benzalkonium chloride. Spheroplasts were more susceptible than whole cells to benzalkonium chloride but not to polymyxin B. Disruption of the outer membrane layer was not by itself sufficient to render P.

Analysis of rifampin disk diffusion and stability in 7H10 agar.

Rifampin was incorporated into Middlebrook 7H10 medium either by adding an aliquot of the antibiotic into melted agar (final concentration 1.0 and 3.0 mug/ml) or by submerging a 5 or 15 mug of rifampin paper disk into 5 ml of melted agar contained in one quadrant of a Felson "X" plate.

Reduced virulence of Pseudomonas aeruginosa grown in the presence of benzalkonium chloride.

Resistant cells of Pseudomonas aeruginosa ATCC 9027 which were grown in the presence of 1 mg of benzalkonium chloride (BC) per ml caused only a mild conjunctivitis when they were dropped onto the scratched corneas of rabbits. In contrast, cells of the BC-sensitive parent strain induced a severe keratoconjunctivitis. In addition, the BC-grown cells also had a reduced capacity to produce kidney infections in mice as compared to the parent strain.

Mesosomes in Pseudomonas aeruginosa.

The use of a combination fixative-staining procedure has allowed a detailed observation of mesosomes in thin sections of Pseudomonas aeruginosa.

Ultrastructural alterations associated with the growth of resistant Pseudomonas aeruginosa in the presence of benzalkonium chloride.

Cells of Pseudomonas aeruginosa resistant to benzalkonium chloride (BC) underwent unique ultrastructural reorganizations when they were grown in the presence of 1 mg of BC/ml. The resistant cells usually contained a single, centrally positioned pseudovacuole. The pseudovacuole was surrounded by a diffuse substance that spread irregularly throughout the cytoplasm.

Resistance of Pseudomonas to quaternary ammonium compounds. II. Cross-resistance characteristics of a mutant of Pseudomonas aeruginosa.

Tube dilution experiments showed that benzalkonium chloride (BC)-resistant mutants of Pseudomonas aeruginosa grown in the presence of 1,000 mug of BC per ml were at least 20 times more sensitive to polymyxin B and colistin sulfate than the BC-sensitive (BCS) parent strain. BCS cells selected for resistance to 500 mug of polymyxin B per ml remained sensitive to BC. There was little difference in the amount of carbenicillin, gentamicin sulfate, or rifampin needed to prevent growth of either the BCS or BC-resistant (BCR) strains.

Resistance of Pseudomonas to quaternary ammonium compounds. I. Growth in benzalkonium chloride solution.

Resistant cells of Pseudomonas aeruginosa and a waterborne Pseudomonas sp. (strain Z-R) were able to multiply in nitrogen-free minimal salts solution containing various concentrations of commercially prepared, ammonium acetate-buffered benzalkonium chloride (CBC), a potent antimicrobial agent. As the CBC concentration increased, growth increased until a point was reached at which the extent of growth leveled off or was completely depressed.

Inhibition of oxygen utilization and destruction of ubiquinone by ultraviolet irradiation of Thiobacillus thiooxidans.

Ultraviolet (UV) irradiation inhibited sulfur oxidation by cells of Thiobacillus thiooxidans. Sulfur-oxidizing activity decreased as the exposure time to UV light increased. A loss of the ability of cells of fix CO(2) paralleled the loss of sulfur-oxidizing activity.

Membrane-associated sulfur oxidation by the autotroph Thiobacillus thiooxidans.

Adair, Frank W. (Rutgers, The State University, New Brunswick, N. J.