Melatonin MT receptor as a novel target in neuropsychopharmacology: MT ligands, pathophysiological and therapeutic implications, and perspectives.

Melatonin (MLT), a neuromodulator mainly acting through two G-protein coupled receptors MT and MT, regulates many brain functions, including circadian rhythms, mood, pain and sleep. MLT and non-selective MT/MT receptor agonists are clinically used in neuropsychiatric and/or sleep disorders. However, the selective roles of the MT and MT receptors need to be clarified.

A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo.

The ability to express exogenous cDNAs while suppressing endogenous genes via RNAi represents an extremely powerful research tool with the most efficient non-transient approach being accomplished through stable viral vector integration. Unfortunately, since traditional restriction enzyme based methods for constructing such vectors are sequence dependent, their construction is often difficult and not amenable to mass production. Here we describe a non-sequence dependent Gateway recombination cloning system for the rapid production of novel lentiviral (pLEG) and retroviral (pREG) vectors.