Publications by authors named "Achim Overbeck"
Temperature influence on the compression and breakage behaviour of yeast cells.
Lett Appl Microbiol
August 2023
Industrial biotechnology uses microbial cells to produce a wide range of products. While the genetic and molecular properties of these organisms are well understood, less is known about their mechanical properties. Previous work has established a test procedure for single yeast cells using a nanoindentation instrument equipped with a flat-punch probe, which allows single cells (Saccharomyces cerevisiae) to be compressed between two parallel surfaces.
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- Understanding the generation and effects of thermomechanical stress during small-scale melt-extrusion is essential for ensuring the stability of protein particles like lysozyme and BSA in PEG matrices.
- The study compared both experimental and numerical methods to analyze how different extrusion designs impact protein stability, finding that continuous screw extrusion (TSE) leads to more protein degradation compared to ram extrusion.
- Results indicated that ram extrusion caused less damage to protein stability, making it the preferred method for producing stable protein-loaded extrudates in small-scale applications.
Functional nanofibrils from globular proteins are usually formed by heating for several hours at pH 2.0, which induces acidic hydrolysis and consecutive self-association. The functional properties of these micro-metre-long anisotropic structures are promising for biodegradable biomaterials and food applications, but their stability at pH > 2.
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