Yerba mate () genome provides new insights into convergent evolution of caffeine biosynthesis.

Yerba mate (YM, ) is an economically important crop marketed for the elaboration of mate, the third-most widely consumed caffeine-containing infusion worldwide. Here, we report the first genome assembly of this species, which has a total length of 1.06 Gb and contains 53,390 protein-coding genes.

Applying nanopore sequencing technology in sp.: a high-quality DNA isolation method for next-generation genomic studies.

Paracoccidioidomycosis is a severe systemic endemic mycosis caused by spp. which mainly affects individuals in Latin America. Progress in genomics has been slow, as evidenced by the incomplete reference databases available.

Patient navigator's role in latent tuberculosis infection at a New York City Health Department Chest Clinic.

Background: Philippines is one of the top ten countries of birth among individuals with tuberculosis in New York City (NYC). The NYC Health Department (HD) screened Filipino-born New Yorkers for latent TB infection (LTBI), but few of those tested positive completed evaluation and treatment.

Objective: To increase the proportion of Filipinos with a positive QuantiFeron-TB Gold Plus (QFT-Plus) complete LTBI evaluation and treatment.

Extrinsic compression of left main coronary artery due to pulmonary artery aneurysm and pulmonary hypertension.

Idiopathic pulmonary arterial hypertension is a serious condition that carries a poor prognosis. While exertional dyspnea is the most common symptom, angina like chest pain, most often due to right ventricle ischemia, may occur at advanced stages. We present a patient with pulmonary hypertension symptomatic for dyspnea and angina in whom computed coronary tomography angiography showed compression of the left main coronary artery by a large pulmonary artery aneurysm.

Perceived Impacts of Urban Gardens and Peer Nutritional Counseling for People Living With HIV in the Dominican Republic.

Objective: Explore participants' perceptions of urban gardens and peer nutritional counseling intervention for people with HIV and food insecurity on antiretroviral therapy in the Dominican Republic.

Methods: Semistructured endline interviews (n = 21) with intervention participants about their perceptions of diet, health, and quality of life. A codebook was applied to verbatim transcripts, and coded data were analyzed using matrices to identify themes.

Non-invasive assessment of sublingual microcirculation using flow derived from green light PPG: evaluation and reference values.

Significance: The study of sublingual microcirculation offers valuable insights into vascular changes and overcomes some limitations of peripheral microcirculation assessment. Videomicroscopy and pulse oximetry have been used to assess microcirculation, providing insights into organ perfusion beyond macrohemodynamics parameters. However, both techniques have important limitations that preclude their use in clinical practice.

Enhanced Method for the Synthesis and Comprehensive Characterization of 1-(4-Phenylquinolin-2-yl)propan-1-one.

We present an enhanced method for synthesizing a novel compound, 1-(4-phenylquinolin-2-yl)propan-1-one (), through the solvent-free Friedländer quinoline synthesis using poly(phosphoric acid) as an assisting agent. The crystal structure of compound is analyzed using FT-IR, and the chemical shifts of its H- and C NMR spectra are measured and calculated using B3LYP/6-311G(d,p), CAM-B3LYP/6-311G(d,p), and M06-2X/6-311G(d,p) basis sets in the gas phase. Additionally, the optimized geometry of quinoline is compared with experimental X-ray diffraction values.

Exploring gender differences in HIV-related stigma and social support in a low-resource setting: A qualitative study in the Dominican Republic.

HIV-related stigma can affect health by compromising coping and social support. Gender differences in stigma experiences and social support are underexplored, particularly in the Caribbean. We conducted semi-structured interviews (N = 32) with patients at two HIV clinics in the Dominican Republic.

Facile synthesis of carbon quantum dot/silver nanocomposite and its antimicrobial, catalytic and sensing applications.

Environmentally benign synthesis of nanocomposite, a pivotal facet of nanotechnology, and gaining prominence due to the increasing demand for facile, sustainable, and safe synthesis methods. The present research reports an facile/cost-effective method for the preparation of carbon dots (CDs) and carbon dot silver nanocomposites ( CD@AgNCs) via a hydrothermal treatment of peanut shells. The well-dispersed and spherical CDs with an average diameter of 5-6 nm were obtained and further employed for the preparation of CD@AgNCs.

The Lambda Variant in Argentina: Analyzing the Evolution and Spread of SARS-CoV-2 Lineage C.37.

The second wave of COVID-19 occurred in South America in early 2021 and was mainly driven by Gamma and Lambda variants. In this study, we aimed to describe the emergence and local genomic diversity of the SARS-CoV-2 Lambda variant in Argentina, from its initial entry into the country until its detection ceased. Molecular surveillance was conducted on 9356 samples from Argentina between October 2020 and April 2022, and sequencing, phylogenetic, and phylogeographic analyses were performed.

Carbon Nanotubes for Confinement-Induced Energetic Nanomaterials.

Oxidized carbon nanotubes obtained by catalytic chemical vapor deposition were filled with an aqueous solution of nano-energetic materials using a very simple impregnation method. The work compares different energetic materials but focuses especially on an inorganic compound belonging to the Werner complexes, [Co(NH)][NO]. Our results show a large increase in released energy upon heating, which we demonstrate to be related to the confinement of the nano-energetic material either directly by filling of the inner channel of carbon nanotubes or to insertion in the triangular channels between adjacent nanotubes when they form bundles.

Biogenic silver based nanostructures: Synthesis, mechanistic approach and biological applications.

The alarming impact of antibiotic resistance sparked the quest for complementary treatments to overcome the confrontation over resistant pathogens. Metallic nanoparticles, especially silver nanoparticles (Ag NPs) have gained a much attention because of their remarkable biological characteristics. Moreover, their medicinal properties can be enhanced by preparing the composites with other materials.

Potential medicinal plants to combat viral infections: A way forward to environmental biotechnology.

The viral diseases encouraged scientific community to evaluate the natural antiviral bioactive components rather than protease inhibitors, harmful organic molecules or nucleic acid analogues. For this purpose, medicinal plants have been gaining tremendous importance in the field of attenuating the various kinds of infectious and non-infectious diseases. Most of the commonly used medicines contains the bioactive components/phytoconstituents that are generally extracted from medicinal plants.

How to Create a Primary Respiratory Care Model.

Respiratory therapists (RTs) are credentialed health professionals who specialize in assessment of pulmonary conditions, performing assessment of pulmonary function and delivering pulmonary therapeutics including aerosol therapy, and noninvasive and invasive mechanical ventilation. Respiratory therapists work closely with various clinicians including physicians, nurses, and therapy staff in a number of different settings including outpatient clinics, long-term facilities, EDs, and ICUs. RTs are integral in the treatment of patients with several acute and chronic conditions.

Catalytic and antimicrobial potential of green synthesized Au and Au@Ag core-shell nanoparticles.

It has been a never-ending quest to design a safe, cost-effective, and environmentally acceptable technology for eliminating contaminants from water and countering antibiotic resistance. Herein, a waste leaf extract from the abundant and renewable plant, Brassica oleracea var. gongylodes, is introduced as a cost-effective and sustainable means to generate gold (Au) and Au@Ag core-shell nanoparticles (NPs).

Transcriptome Dynamics of Rooting Zone and Leaves during In Vitro Adventitious Root Formation in .

Wood properties and agronomic traits associated with fast growth and frost tolerance make a valuable forest alternative. However, the rapid age-related decline in the adventitious root (AR) formation (herein, meaning induction, initiation, and expression stages) limits its propagation. We analyzed transcriptomic profile variation in leaves and stem bases during AR induction of microcuttings to elucidate the molecular mechanisms involved in AR formation.

Preliminary Effects of an Urban Gardens and Peer Nutritional Counseling Intervention on HIV Treatment Adherence and Detectable Viral Load Among People with HIV and Food Insecurity: Evidence from a Pilot Cluster Randomized Controlled Trial in the Dominican Republic.

A pilot cluster randomized controlled trial involving two HIV clinics in the Dominican Republic assessed preliminary efficacy of an urban garden and peer nutritional counseling intervention. A total of 115 participants (52 intervention, 63 control) with moderate or severe food insecurity and sub-optimal antiretroviral therapy (ART) adherence and/or detectable viral load were assessed at baseline, 6- and 12-months. Longitudinal multivariate regression analysis controlling for socio-demographics and accounting for serial cluster correlation found that the intervention: reduced the prevalence of detectable viral load by 20 percentage points at 12 months; reduced any missed clinic appointments by 34 and 16 percentage points at 6 and 12 months; increased the probability of "perfect" ART adherence by 24 and 20 percentage points at 6 and 12 months; and decreased food insecurity at 6 and 12 months.

Establishing a Core Outcome Measure for Peritoneal Dialysis-related Peritonitis: A Standardized Outcomes in Nephrology-Peritoneal Dialysis Consensus Workshop Report.

Introduction: Peritoneal dialysis (PD)-related peritonitis is one of the leading causes of discontinuation of PD and is considered a critically important outcome for patients on PD. However, there is no universally accepted method of measuring this outcome in clinical trials.

Methods: We convened an online consensus workshop to establish a core outcome measure for PD-related peritonitis in clinical trials.

The Role of Antibodies in the Treatment of SARS-CoV-2 Virus Infection, and Evaluating Their Contribution to Antibody-Dependent Enhancement of Infection.

Antibodies play a crucial role in the immune response, in fighting off pathogens as well as helping create strong immunological memory. Antibody-dependent enhancement (ADE) occurs when non-neutralising antibodies recognise and bind to a pathogen, but are unable to prevent infection, and is widely known and is reported as occurring in infection caused by several viruses. This narrative review explores the ADE phenomenon, its occurrence in viral infections and evaluates its role in infection by SARS-CoV-2 virus, which causes coronavirus disease 2019 (COVID-19).

Shock determination in a two-stage decision-making model: The case of COVID-19 in Colombia.

The contributions of this paper are as follows: (a) proposing a two-stage model to study whether an event has temporary or permanent effects on the probability of choosing a good within a market basket that traditional decision theory cannot explain and (b) studying the effects of COVID-19 on consumers' decisions in Colombia. Findings suggest that the pandemic has transitory effects on preferences in the short run. If it lasts longer, it could induce to permanent changes.

Mucosal and systemic immune responses following mucosal immunisation of tetanus toxoid entrapped in lipid nanoparticles prepared by microwave reactor.

In this study, the use of a microwave reactor, which allowed high input of energy into a pressurised system in a short period of time, was investigated for preparation of lipid nanoparticles (LNPs). The aim was to optimise the formulation process by reducing manufacturing time. Two types of LNPs were prepared; non-ionic surfactant vesicles (NISV) and bilosomes (modified NISV incorporating bile salts), with a model antigen (tetanus toxoid, TT) and the immune response induced after mucosal (nasal and oral, respectively) administration was assessed.

Cost-Effective Method to Perform SARS-CoV-2 Variant Surveillance: Detection of Alpha, Gamma, Lambda, Delta, Epsilon, and Zeta in Argentina.

SARS-CoV-2 variants with concerning characteristics have emerged since the end of 2020. Surveillance of SARS-CoV-2 variants was performed on a total of 4,851 samples from the capital city and 10 provinces of Argentina, during 51 epidemiological weeks (EWs) that covered the end of the first wave and the ongoing second wave of the COVID-19 pandemic in the country (EW 44/2020 to EW 41/2021). The surveillance strategy was mainly based on Sanger sequencing of a Spike coding region that allows the identification of signature mutations associated with variants.

Toe Support Pattern as a New Predictive Factor Associated with Falls in Older People with Psychiatric Disorders.

Background: Maintaining autonomy is one of the principal objectives for seniors and people with psychiatric disorders. Podiatric medical care can help them maintain autonomy. This work aimed to characterize and quantify the support of the toes in a psychiatric population by analyzing the influence of psychotropic medications and toe and foot support parameters on the prevalence of falls.