Transport characteristics of glutamine in human intestinal brush-border membrane vesicles.

Transport of glutamine (Gln) across the brush-border membrane of the human intestine was examined using the brush-border membrane vesicle (BBMV) technique. Osmolarity and temperature studies indicate that the uptake of Gln by BBMV is mostly the result of transport of the substrate into the intravesicular space. Transport of Gln was Na+ gradient dependent (out greater than in) with a distinct "overshoot" phenomenon.