Disruption of long-chain base hydroxylation alters growth and impacts sphingolipid synthesis in .

Sphingolipids have roles as membrane structural components and as bioactive molecules in plants. In , 4-hydroxysphinganine (phytosphingosine, t18:0) is the predominant sphingolipid long-chain base (LCB). To assess the functional significance of t18:0, CRISPR-Cas9 mutagenesis was used to generate mutant lines lacking the sole () gene encoding the hydroxylase responsible for converting sphinganine (d18:0) to t18:0.