Occurrence of Plasmid-Mediated Quinolone Resistance and Carbapenemase-Encoding Genes in Isolates from Nosocomial Patients in Aguascalientes, Mexico.

is a leading cause of healthcare-associated infections, which are related to substantial morbidity and mortality. The incidence of Plasmid-Mediated Quinolone Resistance (PMQR) determinants has been previously reported in this bacterium. However, there is limited information regarding the presence of PMQR and carbapenemase-encoding genes simultaneously.

Expression analysis of defense signaling marker genes in Capsicum annuum in response to phytohormones elicitation.

Background: To tolerate biotic stress, plants employ phytohormones such as jasmonic acid (JA), salicylic acid (SA), and ethylene (ET) to regulate the immune response against different pathogens. Phytohormone-responsive genes, known as "Defense signaling marker genes," are used to evaluate plant disease resistance during pathogen infection. Most information on these marker genes derives from studies on the model plant Arabidopsis thaliana.

A perspective on nanomaterials against Campylobacter jejuni biofilm - New control strategies.

Campylobacter jejuni - a Gram-negative bacterium - is considered the fourth cause of diarrheic diseases that can form biofilms (mono and multi-species) or colonize pre-existing biofilms adhering to both, inert or biotic surfaces; its biofilms contribute to transmission through the food chain and survival under harsh environmental conditions. Thus, developing alternatives against this pathogen is compulsory. Nanomaterials have revolutionized the way of fighting infections related to biofilms due to their unique properties compared to traditional antibiotics.

Effect of BvAgNP on growth, development, and glyoxalase gene expression analysis in Mammillaria bombycina and Selenicereus undatus.

Background: Silver nanoparticles (AgNPs) have been used in plant tissue culture as growth stimulants, promoting bud initiation, germination, and rooting. In prior studies, AgNPs were synthesized and characterized by green synthesis using extracts from Beta vulgaris var. cicla (BvAgNP), and their functionality as seed disinfectant and antimicrobial was verified.

Bacterial communities of the psyllid pest (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico.

Background: The psyllid, , is an insect vector of ' Liberibacter' causing "Zebra chip" disease that affects potato and other crops worldwide. In the present study, we analyzed the bacterial communities associated with the insect vector central haplotype of tomato crop fields in four regions from Mexico.

Methods: PCR was used to amplify the mitochondrial () and then analyze the single nucleotide polymorphisms (SNP) and phylogenetic analysis for haplotype identification of the isolated .

Bacterial Strains from Saline Environment Modulate the Expression of Saline Stress-Responsive Genes in Pepper ().

Salinity stress is one of the most important problems in crop productivity. Plant growth-promoting bacteria (PGPB) can also confer stress tolerance in plants under saline soil conditions. In a previous work, it was reported that bacteria strains isolated from hypersaline sites mitigated salt stress in chili pepper ( var.

De Novo Transcriptome of (Cactaceae) under In Vitro Conditions and Identification of Glyoxalase Genes.

is a cactus distributed in the central region of Mexico. Cactaceae have the particularity of surviving drought and high temperatures, which is why in vitro propagation studies have been carried out successfully to preserve this species and use it as a study model in cacti. In this contribution, a de novo transcriptome of was produced under in vitro conditions for the identification and expression of genes related to abiotic stress.

Habanero pepper () adaptation to water-deficit stress in a protected agricultural system.

Drought is one of the major factors limiting global crop yield. In Mexico, agriculture is expected to be severely affected by drought. The Capsicum genus has several crop species of agricultural importance.

Characterization of microbial communities from rumen and large intestine of lactating creole goats grazing in arid plant communities.

Arid plant communities provide variable diets that can affect digestive microbial communities of free-foraging ruminants. Thus, we used next-generation sequencing of 16S and 18S rDNA to characterize microbial communities in the rumen (regurgitated digesta) and large intestine (faeces) and diet composition of lactating creole goats from five flocks grazing in native plant communities in the Sonoran Desert in the rainy season. The bacterial communities in the rumen and large intestine of the five flocks had similar alpha diversity (Chao1, Shannon, and Simpson indices).

Surviving on Environmental Multi-Species Biofilms in Swine Farms.

is the etiologic agent of porcine contagious pleuropneumonia, an important respiratory disease for the pig industry. has traditionally been considered an obligate pig pathogen. However, its presence in the environment is starting to be known.

Biochemical characterization of LysVpKK5 endolysin from a marine vibriophage.

Endolysins have been proposed as a potential antibacterial alternative for aquaculture, especially against Vibrio; the bacterial-agents that most frequently cause disease. Although multiple marine vibriophages have been characterized to date, research on vibriophage endolysins is recent. In this study, biochemical characterization of LysVpKK5 endolysin encoded by Vibrio parahaemolyticus-infecting VpKK5 phage was performed.

Bacterial biofilm-derived antigens: a new strategy for vaccine development against infectious diseases.

Introduction: Microorganisms can develop into a social organization known as biofilms and these communities can be found in virtually all types of environment on earth. In biofilms, cells grow as multicellular communities held together by a self-produced extracellular matrix. Living within a biofilm allows for the emergence of specific properties for these cells that their planktonic counterparts do not have.

Growth effects in oregano plants ( L.) assessment through inoculation of bacteria isolated from crop fields located on desert soils.

Bacteria can establish beneficial interactions with plants by acting as growth promoters and enhancing stress tolerance during plant interactions. Likewise, bacteria can develop multispecies communities where multiple interactions are possible. In this work, we assessed the physiological effects of three bacteria isolated from an arid environment (, , and ) applied as single species or as a consortium on oregano ( L.

Molecular characterization and phylogenetic analysis of a isolate from Baja California Sur, Mexico.

Background: The begomovirus, squash leaf curl virus (SLCuV) is one of the causal agents of squash leaf curl (SLC) disease, which is among the most destructive diseases of cucurbit crops in tropical, subtropical, and semiarid regions worldwide. This disease was originally reported in the American continent with subsequent spread to the Mediterranean basin. Up to now, SLCuV has only been detected by PCR in Mexico.

Incorporation of in Preformed Biofilms by Isolated From Drinking Water of Swine Farms.

, the etiological agent of porcine pleuropneumonia, represents one of the most important health problems in the swine industry worldwide and it is included in the porcine respiratory disease complex. One of the bacterial survival strategies is biofilm formation, which are bacterial communities embedded in an extracellular matrix that could be attached to a living or an inert surface. Until recently, was considered to be an obligate pathogen.

Identification of a 'Candidatus Phytoplasma hispanicum'-related strain, associated with yellows-type diseases, in smoke-tree sharpshooter (Homalodisca liturata Ball).

The 16SrXIII group from phytoplasma bacteria were identified in salivary glands from Homalodisca liturata, which were collected in El Comitán on the Baja California peninsula in Mexico. We were able to positively identify 15 16S rRNA gene sequences with the corresponding signature sequence of 'CandidatusPhytoplasma' (CAAGAYBATKATGTKTAGCYGGDCT) and in silico restriction fragment length polymorphism (RFLP) profiles (F value estimations) coupled with a phylogenetic analysis to confirm their relatedness to 'CandidatusPhytoplasma hispanicum', which in turn belongs to the 16SrXIII group. A restriction analysis was carried out with AluI and EcoRI to confirm that the five sequences belongs to subgroup D.

New trends in innovative vaccine development against Actinobacillus pleuropneumoniae.

Actinobacillus pleuropneumoniae is the etiological agent of porcine pleuropneumonia, a respiratory disease leading to severe economic losses in the swine industry. The most widely used commercial vaccines are bacterins comprising inactivated whole cells of A. pleuropneumoniae but these have only been partially effective in preventing disease.

Actinobacillus pleuropneumoniae biofilms: Role in pathogenicity and potential impact for vaccination development.

Actinobacillus pleuropneumoniae is a Gram-negative bacterium that belongs to the family Pasteurellaceae. It is the causative agent of porcine pleuropneumonia, a highly contagious respiratory disease that is responsible for major economic losses in the global pork industry. The disease may present itself as a chronic or an acute infection characterized by severe pathology, including hemorrhage, fibrinous and necrotic lung lesions, and, in the worst cases, rapid death.

Auxotrophic Actinobacillus pleurpneumoniae grows in multispecies biofilms without the need for nicotinamide-adenine dinucleotide (NAD) supplementation.

Background: Actinobacillus pleuropneumoniae is the etiologic agent of porcine contagious pleuropneumonia, which causes important worldwide economic losses in the swine industry. Several respiratory tract infections are associated with biofilm formation, and A. pleuropneumoniae has the ability to form biofilms in vitro.

Waterborne pathogens: detection methods and challenges.

Waterborne pathogens and related diseases are a major public health concern worldwide, not only by the morbidity and mortality that they cause, but by the high cost that represents their prevention and treatment. These diseases are directly related to environmental deterioration and pollution. Despite the continued efforts to maintain water safety, waterborne outbreaks are still reported globally.

Detection of Actinobacillus pleuropneumoniae in drinking water from pig farms.

Actinobacillus pleuropneumoniae is the aetiological agent of porcine pleuropneumonia and is normally transmitted by aerosols and direct contact between animals. A. pleuropneumoniae has traditionally been considered an obligate pathogen of pigs and its presence in the environment has yet to be investigated.