Publications by authors named "Abodolrazagh Hashemi"
Herein, we report repeated isolation of Mycobacterium europaeum from the sputum samples of an Iranian human immunodeficiency virus-infected patient and a cystic fibrosis patient with chronic pulmonary disease. To our knowledge, this is the first isolation of M. europaeum from human clinical specimens in Asia to be reported.
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- A new species of rapidly growing scotochromogenic mycobacteria has been isolated from clinical samples across six countries, including Iran and Italy.
- Characterization of the strains showed they produce orange-colored colonies and contain unique DNA sequences in key genetic regions.
- The species has been named Mycobacterium iranicum sp. nov., with its type strain designated as M05(T), highlighting its origin from Iran.
Article Synopsis
- The study aimed to use a simpler method called RAPD analysis to examine the diversity of Mycobacterium tuberculosis strains from Iran.
- Researchers analyzed 96 strains collected from patients in Isfahan and Tehran, confirming their identity using conventional and molecular methods.
- The results showed significant variation among the strains, particularly with the primer INS-2, which identified 31 distinct types, suggesting RAPD analysis is a quick and effective way to study tuberculosis diversity in resource-limited settings.
Article Synopsis
- - The text discusses the first reported case of Mycobacterium monacense isolated from the sputum of a patient in Iran.
- - The identification of the bacteria was confirmed using both conventional and molecular techniques.
- - It highlights that M. monacense infections can occur outside of developed countries, indicating a broader geographic distribution.
Objectives: In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria.
View Article and Find Full Text PDFIn developing countries where tuberculosis is still a health challenge, the prevalence of nontuberculous mycobacterial diseases is expected to rise as medical conditions that compromise the immune system become more widespread. In the current study, we aimed to determine the presence and diversity of nontuberculous mycobacteria (NTM) causing infections in Iranian patients. Sixty-seven clinical NTM isolates were identified using conventional and molecular methods, including PCR-restriction fragment length polymorphism analysis (PRA) and 16S rRNA sequencing.
View Article and Find Full Text PDFWe herein present the third set of documented clinical Mycobacterium setense cases. Three clinically unrelated isolates were identified and characterized using various key conventional and molecular diagnostic tests. Phenotypic and molecular data analysis, particularly 16S rDNA, hsp65, and rpoB sequencing, provided evidence of M.
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