Improvement of Monacolin K and Pigment Production in by 5-Azacytidine.

species are known to produce various secondary metabolites with polyketide structures, including Monacolins, pigments, and citrinin. This study investigates the effects of 5-azacytidine on M1 and RP2. The dry weight, red, yellow, and orange pigment values, and Monacolin K yield of both strains were measured, and their hyphae observed through electron microscopy.

Functional Verification of Transcription Factor comp54181_c0 in Monascus purpureus.

Monacolin K is a valuable secondary metabolite produced after a period of fermentation by Monascus purpureus; however, our current understanding of the regulatory mechanisms of its synthesis remains incomplete. This study conducted functional analysis on the key transcription factor, comp54181_c0, that is involved in the synthesis of monacolin K in Monascus. Mutant strains with either knockout or overexpression of comp54181_c0 were constructed using CRISPR/Cas9.

Comprehensive analysis of scRNA-Seq and bulk RNA-Seq reveals ubiquitin promotes pulmonary fibrosis in chronic pulmonary diseases.

It is estimated that there are 544.9 million people suffering from chronic respiratory diseases in the world, which is the third largest chronic disease. Although there are various clinical treatment methods, there is no specific drug for chronic pulmonary diseases, including chronic obstructive pulmonary disease (COPD), interstitial lung disease (ILD) and idiopathic pulmonary fibrosis (IPF).

Mutation Breeding of to Produce a High Yield of Orange Pigment and Low Citrinin Content Using the ARTP Method.

is a filamentous fungus with a long history of application in China, which can produce a variety of secondary metabolites, including Monascus red pigments, orange pigments, yellow pigments, and citrinin. There is widespread attention being paid to natural pigments because of their safety. Among the many natural pigments, orange pigment has a wide range of applications because of its unique color, but current production levels in the orange pigment industry are limited to a certain extent due to the insufficiently wide range of sources and low production.

Aquaporin 1 aggravates lipopolysaccharide-induced macrophage polarization and pyroptosis.

Acute respiratory infections (ARIs) are associated with high mortality and morbidity. Acute lung injury (ALI) is caused by the activation of immune cells during ARIs caused by viruses such as SARS-CoV-2. Aquaporin 1 (AQP1) is distributed in a variety of immune cells and is related to the occurrence of ALI, but the mechanism is not clear.

Occurrence, Risk Implications, Prevention and Control of CIT in Cheese: A Review.

is a filamentous fungus that has been used in the food and pharmaceutical industries. When used as an auxiliary fermenting agent in the manufacturing of cheese, cheese is obtained. Citrinin (CIT) is a well-known hepatorenal toxin produced by that can harm the kidneys structurally and functionally and is frequently found in foods.

Speech Features as Predictors of Momentary Depression Severity in Patients With Depressive Disorder Undergoing Sleep Deprivation Therapy: Ambulatory Assessment Pilot Study.

Background: The use of mobile devices to continuously monitor objectively extracted parameters of depressive symptomatology is seen as an important step in the understanding and prevention of upcoming depressive episodes. Speech features such as pitch variability, speech pauses, and speech rate are promising indicators, but empirical evidence is limited, given the variability of study designs.

Objective: Previous research studies have found different speech patterns when comparing single speech recordings between patients and healthy controls, but only a few studies have used repeated assessments to compare depressive and nondepressive episodes within the same patient.

Quantum Energy Current Induced Coherence in a Spin Chain under Non-Markovian Environments.

We investigate the time-dependent behaviour of the energy current between a quantum spin chain and its surrounding non-Markovian and finite temperature baths, together with its relationship to the coherence dynamics of the system. To be specific, both the system and the baths are assumed to be initially in thermal equilibrium at temperature Ts and Tb, respectively. This model plays a fundamental role in study of quantum system evolution towards thermal equilibrium in an open system.

Effect of polysaccharide on immunomodulatory activity in mice.

polysaccharides (MCPs) have been reported to exert beneficial roles, such as disease healing, in medicine and pharmacy. However, little is known about their effects on immunomodulation The present study aimed to explore the possible effects of polysaccharide (MCP) on the immunomodulatory activity of mice lymphocytes. To this aim, male BALB/c mice aged 6-8 weeks were assigned to the following six experimental groups: i) Normal (NG); ii) model (MG); iii) positive (PG); iv) MCP low-dose (MLG); v) MCP medium-dose (MMG); and vi) MCP high-dose (MHG).

Testicular AQP1 expression in a rat model of testicular Ischemia-Reperfusion injury.

Unlabelled: Aquaporin 1 (AQP1) is the archetype of all aquaporins and involved in rapid cellular water fluxes and cell volume regulation.

An Objective: This study was conducted for the investigation of AQP1 expression in normal testicular tissues and those with I/R injury in a rat model.

Study Design: A TT rat model was established using male Wister rats (4 weeks old, 180-220 g), and AQP1 distribution in the testicular tissues was detected by immunohistochemistry.

Magnetic resonance imaging of the Lisfranc ligament.

Background: The Lisfranc joint has complex structures, and articular surfaces overlap on conventional X-ray radiographs. Hence, there is no available auxiliary examination for diagnosing related injuries. At present, few studies on the imaging of Lisfranc ligaments have been reported, and related imaging data are rare.

Changes in mucin 1 expression in a rat model of allergic airway inflammation.

Purpose: To explore the distribution and expressional changes of mucin 1 (Muc1) in airway of rats with allergic airway inflammation.

Materials And Methods: Ovalbumin (OVA) was used to induce acute allergic inflammation in male Wistar rats. The distributions and expressions of Muc1 in lungs of normal and model rats were assessed by immunohistochemical staining and western blotting, respectively.

[Clinical application of retroperitoneal laparoscopic surgery combined with mini-flank incision "hybrid surgery" for partial nephrectomy of complex renal tumors].

Objective: To evaluate the clinical effect and safety of retroperitoneal laparoscopic surgery combined with mini-flank incision "hybrid surgery" for partial nephrectomy of complex renal tumors.

Methods: Between April 2015 and December 2016, the clinical data from 16 patients with complex renal tumors who underwent the "hybrid surgery", including 10 males and 6 females, were retrospectively reviewed. The average age was (50.

Superconductivity in KCa2Fe4As4F2 with Separate Double Fe2As2 Layers.

We report the synthesis, crystal structure, and physical properties of a quinary iron arsenide fluoride, KCa2Fe4As4F2. The new compound crystallizes in a body-centered tetragonal lattice (space group I4/mmm, a = 3.8684(2) Å, c = 31.

Comparison of drug-eluting balloon versus drug-eluting stent in patients with in-stent restenosis: insight from randomized controlled trials.

Backgrounds: In-stent restenosis (ISR) remains an important issue even in the current drug-eluting stent (DES) era. We performed a meta-analysis to assess the clinical efficacy and safety of drug-eluting balloon (DEB) as compared with DES for the treatment of ISR.

Methods: The published literature was scanned by formal searches of electronic databases from January 2005 to February 2014.

Design and synthesis of a new layered thermoelectric material LaPbBiS3O.

A new quinary oxysulfide LaPbBiS3O was designed and successfully synthesized via a solid-state reaction in a sealed evacuated quartz tube. This material, composed of stacked NaCl-like [M4S6] (where M = Pb, Bi) layers and fluorite-type [La2O2] layers, crystallizes in the tetragonal space group P4/nmm with a = 4.0982(1) Å, c = 19.

Drug-coated balloon angioplasty for drug-eluting stent restenosis: insight from randomized controlled trials.

Background: The best treatment option for drug-eluting stent (DES) restenosis has not been established. We performed a meta-analysis to assess the clinical efficacy of drug-coated balloon (DCB) for the treatment of DES restenosis.

Methods: Trials were identified through a literature search from January 2005 through April 2014.

Paternal and maternal genetic analysis of a desert Keriyan population: Keriyans are not the descendants of Guge Tibetans.

The Keriyan people live in an isolated village in the Taklimakan Desert in Xinjiang, Western China. The origin and migration of the Keriyans remains unclear. We studied paternal and maternal genetic variance through typing Y-STR loci and sequencing the complete control region of the mtDNA and compared them with other adjacent populations.

Expression of aquaporins in the lungs of mice with acute injury caused by LPS treatment.

The aim of the present study was to investigate the expression of aquaporin 1 (AQP1) and AQP5 in the lungs of mice with acute injury induced by LPS treatment. In the study, the concentrations of cytokines were all significantly increased in the BALF of mice received LPS at 12h and 24h (P<0.001).

Genetic polymorphism of 17 Y chromosomal STRs in Kazakh and Uighur populations from Xinjiang, China.

To evaluate the utility of Y-STR data for DNA testing in two ethnic populations of Xinjiang province, a sample of 338 subjects (121 Kazakhs and 217 Uighurs) was tested. In the Kazakh and Uighur populations, the haplotype diversity was 0.868 and 0.

Growth and characterizations of BaTiFeAsO single crystals.

Single crystals of a new iron-based superconductor BaTiFeAsO have been grown successfully via a BaAs-flux method in a sealed evacuated quartz tube. Bulk superconductivity with ∼ 21.5 K was demonstrated in resistivity and magnetic susceptibility measurements after the as-grown crystals were annealed at 500 °C in vacuum for a week.

Genetic diversities of cytochrome B in Xinjiang Uyghur unveiled its origin and migration history.

Background: Uyghurs are one of the many populations of Central Eurasia that is considered to be genetically related to Eastern and Western Eurasian populations. However, there are some different opinions on the relative importance of the degree of Eastern and Western Eurasian genetic influence. In addition, the genetic diversity of the Uyghur in different geographic locations has not been clearly studied.