Ras Association Domain Family 1A Gene Promoter Methylation as a Biomarker for Chronic Viral Hepatitis C-related Hepatocellular Carcinoma.

Background One of the most prevalent aberrant epigenetic modifications found in hepatocellular carcinoma (HCC) is abnormal DNA methylation. Our study aimed to evaluate serum Ras association domain family 1A (RASSF1A) gene promoter methylation in patients with chronic viral hepatitis C (HCV)-associated liver cirrhosis with and without HCC as a potential new marker for the early detection of HCC. Methodology The 60 participants who participated in the trial were divided into the following three groups: 20 patients with newly diagnosed primary HCC on top of HCV-related liver cirrhosis, 20 patients with HCV-related liver cirrhosis, and 20 age- and sex-matched healthy individuals as a control group.

HOXA9 gene promotor methylation and copy number variation of SOX2 and HV2 genes in cell free DNA: A potential diagnostic panel for non-small cell lung cancer.

Background: Most cases of lung cancer are diagnosed at advanced stage. Detection of genetic and epigenetic markers in cell-free DNA (cfDNA) is a promising tool for the diagnosis of lung cancer at an early stage. The aim of this study was to identify non-invasive diagnostic markers in cell free DNA (cfDNA) for non-small cell lung cancer (NSCLC) as it is the most common type of lung cancer.

HIRA directly targets the enhancers of selected cardiac transcription factors during in vitro differentiation of mouse embryonic stem cells.

HIRA is a histone chaperone known to modulate gene expression through the deposition of H3.3. Conditional knockout of Hira in embryonic mouse hearts leads to cardiac septal defects.

The Expression of Activating Receptor Gene of Natural Killer Cells () in Patients with Type 1 Diabetes Mellitus (T1DM).

Objectives: To identify the possible role of natural killer (NK) cells in the pathogenesis of type 1 diabetes mellitus (T1DM) through studying the expression of the gene, which encodes the NK cell activating receptor (NKG2E).

Methods: This study was conducted at Alexandria University Children's Hospital from April to October 2015. The study was conducted with 30 newly diagnosed T1DM patients (15 males and 15 females), aged 7-13 years (10.

Relation of middle molecules levels and oxidative stress to erythropoietin requirements in high-flux versus low-flux hemodialysis.

The objective of this study is to investigate the serum beta-2-microglobulin (B2MG) and advanced oxidation protein products (AOPP) as middle molecule uremic toxins and protein carbonyl (PCO) as oxidative stress marker in uremic patients undergoing high-flux versus low-flux hemodialysis (HD) and to correlate their levels to the erythropoietin requirements for those patients. Twenty patients on chronic low-flux HD were recruited in the study. At the start of the study, all patients underwent high-flux HD for eight weeks, followed by low-flux HD for two weeks as a washout period.

MicroRNA 146a expression in rheumatoid arthritis: association with tumor necrosis factor-alpha and disease activity.

Aim: MicroRNAs (miRNAs) regulate the expression of many genes and may be involved in regulating the immune response. Expression of microRNA 146a (miRNA 146a) in peripheral blood mononuclear cells was studied by real-time polymerase chain reaction in 70 patients with rheumatoid arthritis (RA), 45 patients with knee osteoarthritis (OA), and 60 healthy controls. Disease activity of RA was assessed using disease activity score (DAS) 28 and physical disability using the Improved Health Assessment Questionnaire.

Methylation status of the gene promoter of cyclin-dependent kinase inhibitor 2A (CDKN2A) in ovarian cancer.

Objective: This study investigated the methylation status of the promoter of the tumor suppressor gene cyclin-dependent kinase inhibitor 2A (CDKN2A) in ovarian cancer.

Design And Methods: CDKN2A methylation was quantified by real-time PCR in ovarian biopsies of 52 patients with ovarian cancer, 43 patients with benign ovarian tumors and 40 patients with benign uterine pathology and healthy ovaries.

Results: CDKN2A methylation was detected in the three groups.

G2019S mutation of the leucine-rich repeat kinase 2 gene in a cohort of Egyptian patients with Parkinson's disease.

Aim: This work investigates the prevalence of G2019S mutation of the leucine-rich repeat kinase 2 (LRRK2) gene in a cohort of Egyptian patients with sporadic Parkinson's disease (PD) and its relation to various features of the disease.

Materials And Methods: The study included 113 patients with sporadic PD and 87 healthy individuals as a control group. Clinical assessment was done using the Unified PD Rating Scale (UPDRS) and staging of PD was done according to Hoehn-Yahr score.

Toll-like receptor 3 gene expression in Egyptian patients with glomerulonephritis and hepatitis C virus infection.

Background: Hepatitis C virus (HCV) infection is associated with both chronic liver disorders and several extrahepatic manifestations including renal disease. Toll-like receptor 3 (TLR3), a component of the innate immune system, is a pathogen recognition receptor that recognizes viral double-stranded RNA.

Objective: This work investigated TLR3 expression in peripheral blood mononuclear cells from HCV-positive patients with glomerulonephritis.

MEFV gene mutations in Egyptian patients with familial Mediterranean fever.

Background: Familial Mediterranean fever (FMF) is an autosomal recessive disorder characterized by recurrent attacks of fever and serositis. The disease affects mainly Mediterranean populations and is caused by mutations in the MEFV gene.

Aim: This work was carried out to identify and determine the frequencies of MEFV gene mutations in Egyptian patients in whom FMF was diagnosed.

Clinical usefulness of basic fibroblast growth factor and E-selectin in patients with rheumatoid arthritis.

Angiogenesis plays an important role in the pathogenesis of rheumatoid arthritis (RA). This study assesses basic fibroblast growth factor (bFGF) as an angiogenic factor and soluble E-selectin (sE-sel) as an angiogenic mediator in RA patients and correlates their levels in serum and synovial fluid (SF) with disease activity, functional status and joint derangement. Thirty RA patients and 15 osteoarthritis (OA) patients were clinically, radiologically and laboratory investigated.