Immunological identification of Fasciola hepatica antigens containing major human T-cell and B-cell epitopes.

Fasciola hepatica whole worm homogenate (Fhwwh) separated fractions were used in enzyme linked immunoelectrotransfer blot (EITB) to identify the antigen(s) which induces antibody formation in human fascioliasis. The immuno-reactive antigens recognized by the infected patients were 25-29 kDa and 12 kDa. Antigens were biochemically purified by model 491-prep cell fraction (BIO-RAD).