Publications by authors named "Abigail S Harris"
Article Synopsis
- MicroRNAs (miRNAs) are crucial noncoding RNAs that help regulate human mRNA protein production after transcription, but their relationship with Argonaute (Ago) proteins is more complex than previously thought.
- There is a significant excess of miRNAs compared to Ago proteins in HeLa cells, suggesting that not all miRNAs are directly bound by Agos, leading to the possibility of miRNA-mRNA interactions without Agos.
- While all human Agos can repress these miRNA-mRNA interactions, only Ago2 has the ability to cleave small interfering RNA-mRNA duplexes, indicating a distinct role of Agos in regulating gene expression.
MicroRNAs (miRNAs) regulate physiological and pathological processes by inducing posttranscriptional repression of target messenger RNAs (mRNAs) via incompletely understood mechanisms. To discover factors required for human miRNA activity, we performed an RNAi screen using a reporter cell line of miRNA-mediated repression of translation initiation. We report that reduced expression of ribosomal protein genes (RPGs) dissociated miRNA complexes from target mRNAs, leading to increased polysome association, translation, and stability of miRNA-targeted mRNAs relative to untargeted mRNAs.
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