Triple-Isotope Tracing for Pathway Discernment of NMN-Induced NAD Biosynthesis in Whole Mice.

Numerous efforts in basic and clinical studies have explored the potential anti-aging and health-promoting effects of NAD-boosting compounds such as nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN). Despite these extensive efforts, our understanding and characterization of their whole-body pharmacodynamics, impact on NAD tissue distribution, and mechanism of action in various tissues remain incomplete. In this study, we administered NMN via intraperitoneal injection or oral gavage and conducted a rigorous evaluation of NMN's pharmacodynamic effects on whole-body NAD homeostasis in mice.