Bicyclo[2.1.1]hexanes via Intramolecular Formal (3+2)-Cycloaddition.

We report a synthesis of bicyclo[2.1.1]hexanes via an intramolecular formal (3+2) cycloaddition of allylated cyclopropanes bearing a 4-nitrobenzimine substituent.

Genome-Wide Quantification of the Effect of Gene Overexpression on Growth.

Recombinant protein production plays an essential role in both biological studies and pharmaceutical production. is one of the most favorable hosts for this purpose. Although a number of strategies for optimizing protein production have been developed, the effect of gene overexpression on host cell growth has been much less studied.