Publications by authors named "Abida Raheem"
Liver tissues, composed of hepatocytes, cholangiocytes, stellate cells, Kupffer cells, and sinusoidal endothelial cells, are differentiated from endodermal and mesodermal germ layers. By mimicking the developmental process of the liver, various differentiation protocols have been published to generate human liver organoids (HLOs) in vitro using induced pluripotent stem cells (iPSCs). However, HLOs derived solely from the endodermal germ layer often encounter technical hurdles such as insufficient maturity and functionality, limiting their utility for disease modeling and hepatotoxicity assays.
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- The study focuses on developing human liver organoids (HLOs) from induced pluripotent stem cells (iPSCs) by mimicking liver development, but traditional HLOs face issues like low maturity and functionality.
- Researchers improved liver organoid maturity by differentiating endodermal progenitor cells (EPCs) and mesoderm-derived vascular progenitor cells (VPCs) together into vascular human liver organoids (vHLOs), which showed better functionality and secretion of essential proteins.
- To further enhance the culture process, vHLOs were 3D bioprinted on a pillar plate platform, resulting in superior maturity and better conditions for high-through