Structurally modified carbon nanotubes as twisted fibers towards electrochemical detection of environmentally hazardous cartap.

Pesticides such as cartap play a crucial role in agriculture, as they are extensively used in controlling pests in crops like sugarcane, rice and vegetables. However, their excessive usage poses major threats to human health, causing neurotoxicity and respiratory disorders. In addition, they harm aquatic ecosystems by disrupting biodiversity.

Comparative metabolomics elucidates the early defense response mechanisms to Plutella xylostella infestation in Brassica napus.

Plutella xylostella (diamondback moth; DBM) is a significant pest of Brassica crops, causing billions of dollars in annual global damage and developing resistance to many insecticides. Climate change is increasing the frequency and severity of infestations by influencing the moth's reproduction and expanding its range, leading to increased crop losses. In this study, we examined the early metabolomic responses of four Brassica napus accessions to DBM infestation, focusing on identifying the metabolic basis of tolerance.

Exploring the oncogenic roles of T-box transcription factor TBX2 and its potential as a therapeutic target.

During embryonic development, the T-box transcription factor TBX2 regulates key processes such as cell fate decisions, migration and tissue morphogenesis, and mutations that lead to reduced TBX2 levels result in developmental abnormalities including congenital heart and skeletal defects. TBX2, on the other hand, is overexpressed in a plethora of cancers where it functions as a powerful oncogene contributing to processes ranging from the bypass of senescence and cell death pathways to the promotion of cell proliferation, and epithelial-to-mesenchymal transition to drive invasion and metastasis. Additionally, TBX2 has been implicated in conferring resistance to anti-cancer drugs resulting in poor therapeutic outcomes.

Prediction of potential drug targets and key inhibitors (ZINC67974679, ZINC67982856, and ZINC05668040) against using integrated computational approaches.

, responsible for flea-borne spotted fever, is a rising zoonotic pathogen posing an increasing global threat due to its expanding geographical distribution. The rise in antibiotic-resistant strains of this pathogen underscores the urgent need for new therapeutic interventions. This study employed a comprehensive subtractive proteomics analysis of the proteome, aiming to identify essential, non-host homologous, and pathogen-specific proteins, which were subsequently evaluated as potential new drug targets.

Medicinal Plants Exhibited Promising Potential to Inhibit Biofilm Formation by Catheter-Associated Bacteria in UTI Patients from Lahore, Pakistan.

The current study was designed to evaluate the antibacterial, antibiofilm, and biofilm inhibitory potential of six medicinal plants, including Trachyspermum ammi, Trigonella foenum-graecum, Nigella sativa, Thymus vulgaris, Terminalia arjuna, and Ipomoea carneaid against catheter-associated bacteria (CAB). Eighteen CAB were identified up to species level using 16S rRNA gene sequencing, viz., Klebsiella pneumoniae, Staphylococcus aureus, and Pseudomonas aeruginosa.

Exploring the Versatility of Azine Derivatives: A Comprehensive Review on Synthesis and Biological Applications.

Organic compounds containing azines, di-imines, or bis-Schiff-bases have two azomethine (-CH=N-) functional groups associated with a bridging component. These constituents have attracted attention from a diversity of disciplines, comprising coordination, medicinal, agriculture chemistry, and organic synthesis, because of their comprehensive chemical reactivity and nature. This study determines common synthetic approaches and various biological and pharmacological activities of several substituted bis-Schiff byproducts.

Tubulin-Binding Region Modulates Cholesterol-Triggered Aggregation of Tau Proteins.

A hallmark of Alzheimer disease (AD) and tauopathies, severe neurodegenerative diseases, is the progressive aggregation of Tau, also known as microtubule-associated Tau protein. Full-length Tau, also known as 2N4R, contains two N-terminal inserts that bind to tubulin. This facilitates the self-assembly of tubulin simultaneously enhancing stability of cell microtubules.

Investigation of and in Pakistan, with notes on the detection of tickborne Rickettsiales.

There is limited information on the occurrence of and ticks, as well as associated and species in Pakistan. Addressing this knowledge gap, the current study aimed at morphomolecular confirmation of these ticks and molecular assessment of associated Rickettsiales bacteria (, and spp.) in Balochistan, Pakistan.

Antibiofilm and Antiquorum Sensing Potential of Pheretima posthum.

Antibiotic resistance is a world wide problem mainly in developing countries. In this work, coelomic fluid (PCF) and paste (PBP) of Pheretima posthuma was assessed for its potential as antibiofilm and anti-quorum sensing (QS) agent against pathogenic bacterial biofilms. PCF and PBP were extracted and biofilm formation time kinetics was examined using crystal violet staining method by utilizing four bacterial isolates in bispecies biofilm (06 combinations; MH5-MH10) and multi species biofilms (05 combinations; MH11-MH15).

Unveiling nature's antidiabetic potential in Berberis orthobotrys seeds for alloxan-induced diabetic rats.

To illustrate the anti-diabetic properties of Berberis orthobotrys seeds was the aim of the current study. After a series of experiments, two doses of aqueous methanolic extract of the seeds were selected i.e.

Peptide-based vaccine design against Hendra virus through immunoinformatics approach.

The Hendra virus (HeV) has resulted in epidemics of respiratory and neurological illnesses in animals. Humans have contracted diseases with high fatality rates as a result of infected domestic animals, but effective vaccinations and therapies are currently not available against HeV. Herein, we analyzed the proteome of HeV and constructed an effective and innovative multi-epitope vaccine using immunoinformatics techniques.

KIF1C activates and extends dynein movement through the FHF cargo adapter.

Cellular cargos move bidirectionally on microtubules by recruiting opposite polarity motors dynein and kinesin. These motors show codependence, where one requires the activity of the other, although the mechanism is unknown. Here we show that kinesin-3 KIF1C acts as both an activator and a processivity factor for dynein, using in vitro reconstitutions of human proteins.

Teratological abnormalities in hard ticks (Acari: Ixodidae) from Pakistan.

Studying teratological abnormalities in ticks are taxonomically important because this poorly understood biological phenomenon causes difficulties in tick's identification. Globally, reports regarding these abnormalities in ticks, reasons of their causes and their impacts are scarce. According to the available published data, there are no studies regarding teratological abnormalities in ticks from Pakistan.

Elucidation of cytotoxicity of α-Synuclein fibrils on immune cells.

Progressive aggregation of α-synuclein (α-Syn), a small cytosolic protein involved in cell vesicle trafficking, in the midbrain, hypothalamus, and thalamus is linked to Parkinson's disease (PD). Amyloid oligomers and fibrils formed as a result of such aggregation are highly toxic to neurons. However, it remains unclear whether amyloid-induced toxicity of neurons is the primary mechanism of the progressive neurodegeneration observed upon PD.

Cobalt-Based Ferrite Modified Carbon Nanotubes Fibers for Flexible and Disposable Microelectrode Toward Electrochemical Glucose Sensing.

Glucose detection is critical in clinical health and the food industry, particularly in the diagnosis of blood sugar levels. Carbon-based fiber materials have recently featured prominently as non-enzymatic electrochemical glucose detectors. Herein, cobalt-based ferrite (CoFeO) in the form of nanoparticles has been successfully fabricated over the carbon nanotubes (CNTs) fiber via a simple hydrothermal process.

L-lysine and surfactant-assisted synthesis of NiCo bimetal oxides for electrochemical water splitting.

In the present study, bimetallic oxides comprising nickel (Ni) and cobalt (Co) were synthesized using a facile hydrothermal method in the presence of CTAB and L-lysine. Their efficacy in catalyzing hydrogen production under alkaline conditions was assessed. Structural, vibrational, and morphological characteristics were analyzed utilizing X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) techniques.

Two new species of Sarothrogammarus (Crustacea, Amphipoda) from Swat Valley, Pakistan.

Two new species of the genus Sarothrogammarus Martynov, 1935 are described and illustrated from Pakistan. Sarothrogammarus miandamensis sp. nov.

Impacts of abiotic factors and pesticide on the development, phenology, and reproductive biology of pink bollworm, (Saunders) (Lepidoptera: Gelechiidae).

The pink bollworm, (Saunders) (Lepidoptera: Gelechiidae) is a serious insect pest of cotton crop. The studies to evaluate the impact of abiotic factors on cotton pests' biology are limited. The current study was undertaken to determine the impact of abiotic factors (temperature, humidity, photoperiod) and an insecticide (lambda-cyhalothrin) on the biological aspects of .

Lumpy Skin Disease: Insights into Molecular Pathogenesis and Control Strategies.

Lumpy skin disease (LSD) is a viral infection that affects buffaloes and cattle across various regions, including both tropical and temperate climates. Intriguingly, the virus-carrying skin sores remain the primary source of infection for extended periods, exacerbated by the abundance of vectors in disease-endemic countries. Recent scientific advances have revealed the molecular aspects of LSD and offered improved vaccines and valuable antiviral targets.

Synthesis, Urease Inhibitory Activity, Molecular Docking, Dynamics, MMGBSA and DFT Studies of Hydrazone-Schiff Bases Bearing Benzimidazole Scaffold.

In this study, eleven hydrazone-Schiff bases bearing benzimidazole moiety were synthesized successfully via three step reactions and structures of these products were deduced by HR-ESI-MS, H-, and C-NMR spectroscopic techniques. Lastly, these derivatives were tested for their in vitro urease inhibitory potential. Six compounds among the series attributed excellent inhibition with IC values of 7.

Cholesterol Accelerates Aggregation of α-Synuclein Simultaneously Increasing the Toxicity of Amyloid Fibrils.

A hallmark of Parkinson disease (PD) is a progressive degeneration of neurons in the substantia nigra pars compacta, hypothalamus, and thalamus. Although the exact etiology of irreversible neuronal degeneration is unclear, a growing body of experimental evidence indicates that PD could be triggered by the abrupt aggregation of α-synuclein (α-Syn), a small membrane protein that is responsible for cell vesicle trafficking. Phospholipids uniquely alter the rate of α-Syn aggregation and, consequently, change the cytotoxicity of α-Syn oligomers and fibrils.