Purification and molecular characterization of IgM in olive barb, Puntius Sarana.

In the present article, immunoglobulin (Ig) of Puntius sarana (a vulnerable medium carp species) was purified by affinity chromatography, characterized, and identified as only IgM type with a native molecular weight of 879 kDa having one heavy (88 kDa) and one light (26 kDa) chain. Further, the developed rabbit antisera against IgM was found to be quite specific to P. sarana IgM and used in ELISA to measure the antibody titer in P.

Sequence information, ontogeny and tissue-specific expression of complement component C3 in Indian major carp, Labeo rohita (Hamilton).

The complement system is one of the first line of immune defence mechanisms as well as a modifier of acquired immunity. C3 is the central complement component primarily synthesized in liver. The local synthesis of C3 in tissues other than liver may play an important role in local inflammatory processes.