Optimized quantification of fatty acids in complex media using advanced analytical techniques.

Rationale: Various medium formulations contain essential fatty acids at concentrations ranging from 10 to 100 mg/L. Accurate and precise lipid measurement in media is crucial for monitoring media quality and conducting studies on lipids in the context of cell culture. This study employed two-dimensional gas chromatography (GC × GC) analyses to offer enhanced resolution, sensitivity, and separation performance compared to GC.

Whole genome sequencing of a novel chitinolytic sp. RB7AG reveals it's chitosan production potential: optimization of the process through Taguchi experimental design.

Unlabelled: This study reports about the De novo whole genome sequencing and analysis of a bacterial isolate sp. Strain. RB7AG, isolated from the sediments of Chilika Lake, Odisha, India.

Phage-tail-like bacteriocins as a biomedical platform to counter anti-microbial resistant pathogens.

Phage Tail Like bacteriocins (PTLBs) has been an area of interest in the last couple of years owing to their varied application against multi-drug resistant (MDR), anti-microbial resistant (AMR) pathogens and their evolutionary link with the dsDNA virus and bacteriophages. PTLBs are defective phages derived from Myoviridae and Siphoviridae phages, PTLBs are distinguished into R-type (Rigid type) characterized by a non-flexible contractile nanotube resembling Myoviridae phage contractile tails, and F-type (Flexible type) with a flexible non-contractile rod-like structure similar to Siphoviridae phages. In this review, we have discussed the structural association, mechanism, and characterization of PTLBs.

Phage delivered CRISPR-Cas system to combat multidrug-resistant pathogens in gut microbiome.

The Host-microbiome interactions that exist inside the gut microbiota operate in a synergistic and abnormal manner. Additionally, the normal homeostasis and functioning of gut microbiota are frequently disrupted by the intervention of Multi-Drug Resistant (MDR) pathogens. CRISPR-Cas (CRISPR-associated protein with clustered regularly interspersed short palindromic repeats) recognized as a prokaryotic immune system has emerged as an effective genome-editing tool to edit and delete specific microbial genes for the expulsion of bacteria through bactericidal action.

Exploration of genomic and functional features of chitinolytic bacterium RC1830, isolated from Chilika Lake, India.

Unlabelled: RC1830 was previously isolated as a novel chitinolytic streptomycete from Chilika Lake, Odisha, India. The strain RC1830 is a representative member of the soil-dwelling, filamentous group that produces the majority of natural antibiotics and secondary metabolites. The objective of this work was to assess the chitin degradation ability and whole-genome sequence of RC1830.

A simple and rapid colorimetric method for the estimation of chitosan produced by microbial degradation of chitin waste.

Chitin is one of the most abundant biopolymers present in the environment. Chitosan being its major derivative can be obtained by hydrolysis of chitin, especially by microbial degradation. Estimation of resulting chitosan produced by chitin degradation is crucial to the process.