Iron(III) ion-assisted transformation of ZIF-67 to a self-supported FeCo-layered double hydroxide for improved water oxidation.

Herein, we have demonstrated Lewis acid Fe(III)-assisted hydroxylation of ZIF-67 to form FeCo-layered double hydroxide (LDH) nanosheets. The catalyst FeCo-LDH produced an excellent water oxidation activity to reach a current density of 20 mA cm at only 190 mV overpotential, superior to that of hydrothermally synthesized LDH with a similar composition.

Effect of strontium doping on the electrochemical pseudocapacitance of YSrMnO perovskites.

Grid-scale bulk energy storage solutions are needed to utilize the full potential of renewable energy technologies. Pseudocapacitive electrochemical energy storage can play a vital role in developing efficient energy storage solutions. The use of perovskites as anion intercalation-type pseudocapacitor electrodes has received significant attention in recent years.

Photocatalytic dye-degradation activity of nano-crystalline Ti M O (M =Ag, Pd, Fe, Ni and = 0, 0.01) for water pollution abatement.

Nanocrystalline metal-ion (M = Fe, Ni, Ag, and Pd) doped and undoped anatase-TiO powders were prepared using a solution combustion method. The photocatalytic degradation of different dyes such as methylene blue (MB), rhodamine B (RB), rhodamine B base (RBB), and thionine acetate (TA) was investigated under UV exposure. The degradation rate of the dyes were found to be better in the case of Ag and Pd doped TiO, whereas Fe and Ni doped TiO showed lower photocatalytic activity compared to undoped TiO nanoparticles.

SrFeO: a novel Fe ↔ Fe redox mediated pseudocapacitive electrode in aqueous electrolyte.

Pseudocapacitors offer both high energy and high power, making them suitable for grid-scale electrochemical energy storage to harness renewable energy produced from sun, wind, and tides. To overcome performance degradation in terms of cycling fading and lower specific capacitance values at high charge/discharge rates of electrochemical pseudocapacitors based on transition-metal oxides, perovskite-structured SrFeO was envisaged as a negative electrode that harnesses the Fe and Fe redox couple to deliver superior performance. SrFeO offers high specific capacitances of 733 F g at a scan rate of 1 mV s and 743 F g at a current density of 1 A g and demonstrates excellent cyclic stability over 2500 repeated cycles with capacitance retention of >92%, achieving 94% coulombic efficiency.

TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts.

Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. TOP1 is also a major driver of endogenous genome instability, particularly when its catalytic intermediate-a covalent TOP1-DNA adduct known as a TOP1 cleavage complex (TOP1cc)-is stabilised. TOP1ccs are highly cytotoxic and a failure to resolve them underlies the pathology of neurological disorders but is also exploited in cancer therapy where TOP1ccs are the target of widely used frontline anti-cancer drugs.

The p97-Ataxin 3 complex regulates homeostasis of the DNA damage response E3 ubiquitin ligase RNF8.

The E3 ubiquitin ligase RNF8 (RING finger protein 8) is a pivotal enzyme for DNA repair. However, RNF8 hyper-accumulation is tumour-promoting and positively correlates with genome instability, cancer cell invasion, metastasis and poor patient prognosis. Very little is known about the mechanisms regulating RNF8 homeostasis to preserve genome stability.

SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication.

The SPRTN metalloprotease is essential for DNA-protein crosslink (DPC) repair and DNA replication in vertebrate cells. Cells deficient in SPRTN protease exhibit DPC-induced replication stress and genome instability, manifesting as premature ageing and liver cancer. Here, we provide a body of evidence suggesting that SPRTN activates the ATR-CHK1 phosphorylation signalling cascade during physiological DNA replication by proteolysis-dependent eviction of CHK1 from replicative chromatin.

Metalloprotease SPRTN/DVC1 Orchestrates Replication-Coupled DNA-Protein Crosslink Repair.

The cytotoxicity of DNA-protein crosslinks (DPCs) is largely ascribed to their ability to block the progression of DNA replication. DPCs frequently occur in cells, either as a consequence of metabolism or exogenous agents, but the mechanism of DPC repair is not completely understood. Here, we characterize SPRTN as a specialized DNA-dependent and DNA replication-coupled metalloprotease for DPC repair.

Procerain B, a cysteine protease from Calotropis procera, requires N-terminus pro-region for activity: cDNA cloning and expression with pro-sequence.

We have previously reported isolation and characterization of a novel plant cysteine protease, Procerain B, from the latex of Calotropis procera. Our initial attempts for active recombinant Procerain B in Escherichiacoli expression system was not successful. The reason for inactive enzyme production was attributed to the absence of 5' pro-region in the Procerain B cDNA that may be involved in proper folding and production of mature active protein.

Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase and catalase enzymes.

Biodegradable polycaprolactone (PCL) nanosphere encapsulating superoxide dismutase (SOD) and catalase (CAT) were successfully synthesized using double emulsion (w/o/w) solvent evaporation technique. Characterization of the nanosphere using dynamic light scattering, field emission scanning electron microscope, and Fourier transform infrared spectroscopy revealed a spherical-shaped nanosphere in a size range of 812 ± 64 nm with moderate protein encapsulation efficiency of 55.42 ± 3.

Immobilization of procerain B, a cysteine endopeptidase, on amberlite MB-150 beads.

Proteases are involved in several crucial biological processes and reported to have important physiological functions. They also have multifarious applications in different industries. The immobilized form of the enzyme further improves its industrial applicability.

cDNA cloning and molecular modeling of procerain B, a novel cysteine endopeptidase isolated from Calotropis procera.

Procerain B, a novel cysteine protease (endopeptidase) isolated from Calotropis procera belongs to Asclepiadaceae family. Purification of the enzyme, biochemical characterization and potential applications are already published by our group. Here, we report cDNA cloning, complete amino acid sequencing and molecular modeling of procerain B.

A novel superoxide dismutase from Cicer arietinum L. seedlings: isolation, purification and characterization.

Superoxide dismutase is an important enzyme with various therapeutic applications. Search of a new source of superoxide dismutase with novel properties has significant importance. The current work reports purification of a novel superoxide dismutase enzyme with unique characteristics.

Glutaraldehyde-activated chitosan matrix for immobilization of a novel cysteine protease, procerain B.

Proteases have several applications in the food industry. We report the immobilization of procerain B, a novel cysteine protease, on glutaraldehyde-activated chitosan beads through covalent attachment. Glutaraldehyde not only serves as a cross-linking agent but also links the procerain B on the surface of bead through primary amine group (either lysine side chain or N-terminal) by Schiff base linkage.

Exploring applications of procerain b, a novel protease from Calotropis procera, and characterization by N-terminal sequencing as well as peptide mass fingerprinting.

Procerain B is a novel cysteine protease isolated from Calotropis procera by our group and published recently. We have further characterized the enzyme by N-terminal sequencing and peptide mass fingerprinting. Procerain B showed maximum sequence similarity (80%) with Asclepain.