Using for High-Yield Production of Challenging Expression Targets and for Protein Perdeuteration.

Production of soluble proteins is essential for structure/function studies; however, this usually requires milligram amounts of protein, which can be difficult to obtain with traditional expression systems. Recently, the Gram-negative bacterium emerged as a novel and alternative host platform for production of proteins in high yields. Here, we used a commercial strain derived from (Vmax X2) to produce soluble bacterial and fungal proteins in milligram scale, which we struggled to achieve in .

Using for high-yield production of challenging expression targets and for protein deuteration.

Production of soluble proteins is essential for structure/function studies, however, this usually requires milligram amounts of protein, which can be difficult to obtain with traditional expression systems. Recently, the Gram-negative bacterium appeared as a novel and alternative host platform for production of proteins in high yields. Here, we used a commercial strain derived from (Vmax X2) to produce soluble bacterial and fungal proteins in milligram scale, which we struggled to achieve in .