Publications by authors named "Abdullah Husain"
We report a method for generating uniform, artifact-free total internal reflection fluorescence (TIRF) excitation via a photonic lantern. Our tapered waveguide, consisting of a multimode input and nine few-mode outputs, enables single-shot TIRF illumination from nine azimuthal directions simultaneously without the introduction of nonstationary devices. Utilizing the photonic lantern for multi-beam excitation provides a low-loss mechanism that supports a wide range of light sources, including high-coherence lasers and various wavelengths in the visible spectrum.
View Article and Find Full Text PDFBackground And Aims: HCC is the most common type of primary liver cancer and is a common malignancy worldwide. About half of all new liver cancers worldwide each year occur in China, including Hong Kong, due to a high prevalence of HBV infection. HBV DNA integrates into the human genome, disrupting the endogenous tumor suppressors/regulatory genes or enhancing the activity of proto-oncogenes.
View Article and Find Full Text PDFArticle Synopsis
- Metabolic reprogramming in cancer is closely linked to hypoxia, which aids rapid tumor growth and reduces oxidative stress; PFKFB, a key glycolytic enzyme, is vital in various cancers but its role in hepatocellular carcinoma (HCC) is not well-studied.
- The researchers analyzed PFKFB4 expression using RNA sequencing and confirmed its up-regulation in HCC through various methods, discovering a correlation with certain genetic mutations and aggressive tumor behavior.
- Functional studies using CRISPR/Cas9 to knock out PFKFB4 demonstrated that its loss impaired HCC development and altered metabolite levels, affecting pathways like glycolysis and the pentose phosphate pathway.
Background And Aims: Understanding the mechanisms of HCC progression and metastasis is crucial to improve early diagnosis and treatment. This study aimed to identify key molecular targets involved in HCC metastasis.
Approach And Results: Using whole-transcriptome sequencing of patients' HCCs, we identified and validated midline 1 interacting protein 1 (MID1IP1) as one of the most significantly upregulated genes in metastatic HCCs, suggesting its potential role in HCC metastasis.
Background & Aims: The highly proliferative nature of hepatocellular carcinoma (HCC) frequently results in a hypoxic intratumoural microenvironment, which creates a therapeutic challenge owing to a lack of mechanistic understanding of the phenomenon. We aimed to identify critical drivers of HCC development and progression in the hypoxic microenvironment.
Methods: We performed integrative analysis of multiple transcriptomic and genomic profiles specific for HCC and hypoxia and identified the Ephrin-A3/Eph receptor A2 (EphA2) axis as a clinically relevant and hypoxia-inducible signalling axis in HCC.
Background: Controversy over the benefits of antioxidants supplements in cancers persists for long. Using hepatocellular carcinoma (HCC) as a model, we investigated the effects of exogenous antioxidants N-acetylcysteine (NAC) and glutathione (GSH) on tumor formation and growth.
Methods: Multiple mouse models, including diethylnitrosamine (DEN)-induced and Trp53KO/C-MycOE-induced HCC models, mouse hepatoma cell and human HCC cell xenograft models with subcutaneous or orthotopic injection were used.
Background And Objectives: Although laser lithotripsy for fragmentation of gallbladder stones has been applied successfully in many clinical situations, this approach has two major limitations: (1) the potential to damage or perforate the bile duct and (2) the efficiency can be affected by the chemical composition of the gallstones. The present study evaluated the use of time-resolved laser-induced fluorescence spectroscopy to classify stone types and distinguish stone from tissue.
Materials And Methods: Ex vivo time-resolved laser-induced fluorescence analysis (excitation wavelength λ = 400 nm and emission wavelength = 450-700 nm) of 54 gallbladder stones and seven gallbladder tissue samples was conducted.