Genetic diversity and infectivity analysis of tomato yellow leaf curl virus Oman and its associated betasatellite.

Tomato yellow leaf curl virus-Oman (TYLCV-OM),  a variant of the Tomato yellow leaf curl virus-Iran (TYLCV-IR) strain, was identified in 2005 as the cause of tomato yellow leaf curl disease (TYLCD) in Oman and is  associated with a betasatellite namely as Tomato leaf curl betasatellite (ToLCB). Surveys were carried out from three diverse Governorates of Oman to investigate the correlation between the betasatellite and the virus. The visual assessment and scoring of infected tomato plants in the field revealed that the association of betasatellite with the disease was highest in Sharqia at 77%, followed by Dakhlia at41% and lowest in Batinah at30% .

Evolutionary Relationships of Omani , .

A study was conducted to investigate the evolutionary relationships of from Oman, in the southeastern part of the Arabian Peninsula. Sequences of the mitochondrial and the nuclear large-subunit ribosomal RNA (LSU rRNA, ) genes were used to investigate the populations of across their distribution in Oman to determine their relationships with other species. Our findings indicate that in Oman is a member of an East African clade, distinct from those in West Africa.

Benzimidazole-acrylonitrile hybrid derivatives act as potent urease inhibitors with possible fungicidal activity.

A series of benzimidazole-acrylonitrile derivatives were designed with urease inhibition approach. were synthesized and characterized (H Nuclear Magnetic Resonance (NMR), C NMR, Mass Spectroscopy (MS) and IR) and subjected to urease inhibition assay using commercial assay kit. A molecular docking study was also performed using Autodock tool software.

Global citrus root microbiota unravels assembly cues and core members.

Introduction: Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.

Methods: In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples.

Pythium and Globisporangium species associated with cucumber rhizosphere causing damping-off and their effects on cucumber seed decay in Oman.

Pythium sensu lato (s.l.) is a pathogenic oomycete.

Rice Weevil ( L.) Gut Bacteria Inhibit Growth of and Degrade Aflatoxin B1.

In this study, bacteria residing in the gut of the rice weevils ( L.) (Coleoptera: Curculionidae) feeding on aflatoxin-contaminated corn kernels were isolated and evaluated for their ability to suppress and to remove/degrade aflatoxin B1 (AFB1). Four morphologically distinct gut-associated bacterial isolates were isolated and identified as (RWGB1), (RWGB2), (RWGB3), and (RWGB4) based on 16S rRNA gene sequence analysis.

Two new species of (Agaricales, Agaricaceae) from Oman, based on morphology and molecular phylogeny.

has small to medium-sized mushrooms and the cap surface with squamulose pellicles, consisting of hymeniform or pseudoparenchymatous cells and yellowish-brown basidiospores. The species of are very similar to those of and it is extremely difficult to differentiate the species of both genera in the field. However, phylogenetically, both the genera are clearly distinct.

Biocontrol Potential of Trichoderma Ghanense and Trichoderma Citrinoviride toward .

-induced damping-off of cucumber is a major constraint to cucumber production in different parts of the world. Although chemical fungicides are used for managing this disease, they have many drawbacks to the environment. The ability of the antagonistic fungi isolated from the rhizosphere and endosphere of and in the control of soilborne pathogen was inspected.

Molecular Signature of a Novel Alternanthera Yellow Vein Virus Variant Infecting the Weed in Oman.

Alternanthera yellow vein virus (AlYVV), a monopartite , has been identified infecting a diverse range of crops and native plants in Pakistan, India, and China. However, distinctive yellow vein symptoms, characteristic of infection, were observed on the weed in Oman, prompting a thorough genomic characterization in this study. The results unveiled a complete genome sequence of 2745 base pairs and an associated betasatellite spanning 1345 base pairs.

Plant-Disease-Suppressive and Growth-Promoting Activities of Endophytic and Rhizobacterial Isolates Associated with .

This study was conducted to investigate the antagonistic potential of endophytic and rhizospheric bacterial isolates obtained from in suppressing and and promoting the growth of cucumber. Molecular identification of bacterial strains associated with confirmed that these strains belong to the , , , , , , , , , and genera. A dual culture assay showed that nine of the bacterial strains exhibited antifungal activity, four of which were effective against both pathogens.

Phylogenetic Relationships of the Mutualistic Fungi Associated with in Oman.

The symbiotic association between fungus-gardening termites and its fungal symbiont has a moderate degree of specificity-although the symbiotic fungi () form a monophyletic clade, there is not a one-to-one association between termite species and their fungus-garden associates. Here, we aim to determine the origin and phylogenetic relationships of in Oman. We used sequences of the internal transcribed spacer region (ITS) and the nuclear large subunit ribosomal RNA (LSU rRNA, 25S) gene and analyzed these with sequences of from other geographic areas.

An Insight into Emerging Begomoviruses and their Satellite Complex causing Papaya Leaf Curl Disease.

Papaya leaf curl disease (PaLCD) was primarily detected in India and causes major economic damage to agriculture crops grown globally, seriously threatening food security. Begomoviruses are communicated by the vector , and their transmission efficiency and persistence in the vector are the highest, exhibiting the widest host range due to adaptation and evolution. Symptoms induced during PaLCD include leaf curl, leaf yellowing, interveinal chlorosis, and reduced fruit quality and yield.

The observation of taxonomic boundaries for the 16SrII and 16SrXXV phytoplasmas using genome-based delimitation.

Within the 16SrII phytoplasma group, subgroups A-X have been classified based on restriction fragment length polymorphism of their 16S rRNA gene, and two species have been described, namely ' Phytoplasma aurantifolia' and '. Phytoplasma australasia'. Strains of 16SrII phytoplasmas are detected across a broad geographic range within Africa, Asia, Australia, Europe and North and South America.

Artificial microRNA-mediated resistance against Oman strain of tomato yellow leaf curl virus.

Tomato yellow leaf curl virus (TYLCV) is a global spreading begomovirus that is exerting a major restraint on global tomato production. In this transgenic approach, an RNA interference (RNAi)-based construct consisting of sequences of an artificial microRNA (amiRNA), a group of small RNA molecules necessary for plant cell development, signal transduction, and stimulus to biotic and abiotic disease was engineered targeting the AC1/Rep gene of the Oman strain of TYLCV-OM. The Rep-amiRNA constructs presented an effective approach in regulating the expression of the Rep gene against TYLCV as a silencing target to create transgenic L.

Relationship between Dubas Bug () Infestation and the Development of Fungal-Induced Leaf Spots in Date Palms ().

The dubas bug () (Hemiptera: Tropiduchidae) is a serious pest in date palms in several date-producing countries, including Oman. Infestation results in a severe reduction in yield and a weakening of date palm growth. In addition, egg laying, which causes injuries to date palm leaves, results in the development of necrotic lesions on the leaves.

Native and Non-Native NAFME Haplotypes Can Be Implicated in Dispersal of Endemic and Introduced Begomoviruses in Oman.

Irrigated agriculture and global trade expansion have facilitated diversification and spread of begomoviruses (), transmitted by the (Gennadius) cryptic species. Oman is situated on major crossroads between Africa and South Asia, where endemic/native and introduced/exotic begomoviruses occur in agroecosystems. The 'B mitotype' belongs to the North Africa-Middle East (NAFME) cryptic species, comprising at least eight endemic haplotypes, of which haplotypes 6 and/or 8 are recognized invasives.

Effectiveness of Endophytic and Rhizospheric Bacteria from spp. in Controlling Damping-Off of Cabbage.

In this study, endophytic and rhizospheric bacteria were isolated from and from Oman, and their in vitro antagonistic activity against was tested using a dual culture assay. The promising strains were tested further for their compatibility and potential for plant growth promotion, biofilm formation, antifungal volatile organic compound (VOC) production, and the biological control of damping-off of cabbage ( L.) under greenhouse conditions.

Antimicrobial Susceptibility of Fresh Produce-Associated Enterobacteriaceae and Enterococci in Oman.

Fresh produce bacteria may have phenotypic and/or genotypic antimicrobial resistance traits that may lead to various consequences on the environment and human health. This study evaluated the susceptibility of fresh produce bacteria (banana, cabbage, capsicum, carrots, cucumber, dates, lettuce, mango, papaya, pomegranate, radish, tomato and watermelon) to chlorhexidine and the antibiotic resistance of enterococci. Eighty-eight Enterobacteriaceae bacteria and 31 enterococci were screened for their susceptibility to chlorhexidine using the broth microdilution method.

Effects of the Rhizosphere Fungus on the Response to Diverse Abiotic Stresses.

This study examined the efficiency of fungal strain () isolated from the rhizosphere of to reduce symptoms of salinity, drought and heavy metal stresses in tomato plants. In vitro evaluation of demonstrated its ability to produce indole-3-Acetic Acid (IAA), ammonia and tolerate varied abiotic stresses on solid media. Tomato plants at 33 days' old, inoculated with or without , were treated with 1.

Salvianolic Acid Modulates Physiological Responses and Stress-Related Genes That Affect Osmotic Stress Tolerance in and .

Drought is a serious threat worldwide to soybean and maize production. This study was conducted to discern the impact of salvianolic acid treatment on osmotic-stressed soybean ( L.) and maize ( L.

Evolutionary Dynamics of Begomoviruses and Its Satellites Infecting Papaya in India.

The genus represents a group of multipartite viruses that significantly damage many agricultural crops, including papaya, and influence overall production. Papaya leaf curl disease (PaLCD) caused by the complex begomovirus species has several important implications and substantial losses in papaya production in many developing countries, including India. The increase in the number of begomovirus species poses a continuous threat to the overall production of papaya.