Psychometric Properties of the Bengali Version Brief Questionnaire on Smoking Urges (QSU-Brief).

The study aimed to investigate the factorial structure, reliability, and validity of the Bengali version of the brief Questionnaire on Smoking Urges (QSU-Brief) tool in a sample of Bangladeshi smokers. The Bengali version QSU-Brief scale's reliability and validity were assessed on the basis of the data provided by 460 Bangladeshi smokers. To substantiate the data reliability, internal consistency and test-retest reliability were measured.

The REFOLD database: a tool for the optimization of protein expression and refolding.

A large proportion of proteins expressed in Escherichia coli form inclusion bodies and thus require renaturation to attain a functional conformation for analysis. In this process, identifying and optimizing the refolding conditions and methodology is often rate limiting. In order to address this problem, we have developed REFOLD, a web-accessible relational database containing the published methods employed in the refolding of recombinant proteins.

Managing and mining protein crystallization data.

The crystallization of macromolecules remains a major bottleneck in structural biology. The routine screening of more than one thousand crystallization conditions and subsequent optimization by fine screening presents a challenge to conventional laboratory notebook keeping. In addition, the development of high-throughput robotic crystallization and imaging systems presents a pressing need for low-cost laboratory information management system (LIMS).

REFOLD: an analytical database of protein refolding methods.

The expression and harvesting of proteins from insoluble inclusion bodies by solubilization and refolding is a technique commonly used in the production of recombinant proteins. To bring clarity to the large and widespread quantity of published protein refolding data, we have recently established the REFOLD database (http://refold.med.