[Age composition of the erythrocyte population, ATP and 2,3-diphosphoglycerate composition in erythrocytes in different forms of hemolytic anemia].

The total erythrocyte population was subdivided into three age groups using as criteria the stability of red blood cells to osmotic shock and the values of glucose-6-phosphate dehydrogenase (G6PD) activity. The "old" erythrocytes constituted about 8%, "mature"--87% and "young"--5% under normal conditions. In hereditary microspherocytosis 70% of erythrocytes possessed a decreased osmotic resistance and higher G6PD activity as compared with normal state.

[Acetylcholinesterase of erythrocytes in various blood diseases].

Appearance of low molecular components of acetylcholinesterase from erythrocyte membranes was found by gel filtration on Sephadex G-200 and polyacrylamide gel disc electrophoresis. Content of these components was distinctly increased within ghe acute period of Marchiafava-Micheli disease.

[ Change in the protein spectrum of the erythrocytic membranes in certain anemias].

Proteins of human erythrocyte membranes were studied in normal state and in different anaemias. After solubilization of cell "shadows" in phenol-urea-acetic acid-water system in membranes there were found, by means of polyacrylamide gel disc electrophoresis, 20 protein fractions with molecular weight from 48000 to 225000; seven of them glycoproteins. In 9 cases out of 36 a complete or partial disappearance of a glycoprotein component with molecular weight of 170000 was observed in erythrocyte membranes from patients with inherited spherocytosis and Markiafava--Mikely disease.