Effects of citric acid modified with fluoride, nano-hydroxyapatite and casein on eroded enamel.

Objectives: The aim of this in vitro study was to evaluate the effects of citric acid containing fluoride, nano-hydroxyapatite, and casein on eroded enamel.

Design: The crowns of 120 extracted bovine incisors were embedded in acrylic resin. An enamel window (2 × 3 mm) was created on the surface.

Cytotoxicity evaluation of luting resin cements on bovine dental pulp-derived cells (bDPCs) by real-time cell analysis.

To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs), Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials were incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival.

Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using realtime cell analysis.

Purpose: To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).

Materials And Methods: Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) for 72 hours according to ISO 10993-5.

Dental approach to erosive tooth wear in gastroesophageal reflux disease.

Background: The duration of gastro-esophageal reflux disease (GERD), the frequency of reflux, the pH and type of acid, and the quality and quantity of saliva affect the severity of dental erosion due to GERD.

Objective: To summarize the diagnostic protocol and treatment of dental erosion due to GERD.

Methods: A Medline literature search was performed to identify articles associated with a dental approach to GERD.

Effect of accelerated aging on the bonding performance of fluoridated adhesive resins.

The purpose of this study was to investigate the dentin bond durability of a one-step, fluoride-containing, glass ionomer-based adhesive system, Reactmer Bond (RB), and that of a two-step, fluoride-containing, self-etch adhesive system, Clearfil Protect Bond (CPB). Enamel was removed from the occlusal surfaces of teeth, and flat dentin surfaces were entirely covered with a composite resin following the application of an adhesive material (n=10). After specimens were sectioned into rectangular sticks of 0.

Cytotoxicity testing of temporary luting cements with two- and three-dimensional cultures of bovine dental pulp-derived cells.

This study evaluated the cytotoxicity of eugenol-containing and eugenol-free temporary luting cements. For cytotoxicity testing, bovine pulp-derived cells transfected with Simian virus 40 Large T antigen were exposed to extracts of eugenol-containing (Rely X Temp E) and eugenol-free (Provicol, PreVISION CEM, and Rely X Temp NE) temporary luting cements for 24 h. The cytotoxicity of the same materials was also evaluated in a dentin barrier test device using three-dimensional cell cultures of bovine pulp-derived cells.

Human and bovine pulp-derived cell reactions to dental resin cements.

Objectives: The objective of this study was to evaluate the cytotoxic reaction of transfected human pulp derived cells (tHPDC) and transfected bovine pulp derived cells (tBPDC) after exposure to resin cements [RelyX UnicemClicker (RX), MaxCem (MC), Panavia F 2.0 (PF), BisCem (BC), and Bistite II DC (BII)] and to compare it to the generation of reactive oxygen species (ROS).

Materials And Methods: Set materials were extracted in culture medium, cell survival as a measure of cytotoxicity was determined photometrically using crystal violet after cells were exposed to the extracts for 24 h.

Cytotoxicity evaluation of dentin bonding agents by dentin barrier test on 3-dimensional pulp cells.

Objective: The aim of this study was to evaluate the effects of 4 dentin-bonding agents on the cell viability of bovine derived cells.

Study Design: Cytotoxicity of dentin-bonding agents (G-Bond [GB], Adper Prompt Self-Etch [APSE], Clearfil DC Bond System [CDCB], and Quadrant University-1-Bond [UB]) was analyzed with a dentin barrier test device using 3-dimensional (3D) pulp cell cultures. A commercially available cell culture perfusion chamber was separated into 2 compartments using a 500 μm dentin disk.

Cytotoxic effects of resin-modified orthodontic band adhesives. Are they safe?

Objective: To evaluate the cytotoxic effects of three different resin-modified orthodontic band adhesives.

Materials And Methods: Three resin-modified orthodontic band adhesives (Bisco Ortho Band Paste LC, Multi-Cure Glass Ionomer Band Cement, and Transbond Plus Light Cure Band Adhesive) were prepared and the samples were extracted in 3 mL of Basal Medium Eagle with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in wells of 96-well dishes and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air.

Cytotoxic effects of orthodontic composites.

Objectives: To evaluate the cytotoxic effects of five different light-cured orthodontic bonding composites.

Materials And Methods: The orthodontic composites Heliosit Orthodontic (Ivoclar), Transbond XT (3M Unitek), Bisco ORTHO (Bisco), Light Bond (Reliance), and Quick Cure (Reliance) were prepared, and the samples were extracted in 3 mL of BME (Basal Medium Eagle) with 10% newborn calf serum for 24 hours. The L929 cells were plated (25,000 cells/mL) in a 96-well dish and maintained in a humidified incubator for 24 hours at 37 degrees C, 5% CO(2), and 95% air.

Effect of artificial aging regimens on the performance of self-etching adhesives.

This in vitro study was performed to compare the microtensile bond strengths (MTBS) of current self-etching adhesives to dentin and to evaluate the effects of artificial aging [(thermocycling (TC) and/or mechanic loading (ML)] on MTBS and on nanoleakage of self-etching adhesives. Two-step (AdheSE Bond, Clearfil Protect Bond, Clearfil SE Bond, Optibond Self-Etch) and one-step (Hybrid Bond, G-bond, Clearfil Tri-S Bond, and Adper Prompt L-Pop) self-etching adhesives were tested. Resin composite build-ups were created, and the specimens were subjected to 10(4) TC, 10(5) ML, and 10(4)/10(5) TC/ML.

Cytotoxicity Evaluation of Self Adhesive Composite Resin Cements by Dentin Barrier Test on 3D Pulp Cells.

Objectives: The aim of this study was to evaluate the effects of five self-etch dental composite resin cements on the cell viability of bovine dental papilla-derived cells.

Methods: The cytotoxicity of composite resin cements (Rely X Unicem Clicker, 3M ESPE; MaxCem; KERR, Panavia F 2.0; Kuraray, BisCem; Bisco and Bistite II DC; Tokuyama) was analyzed in a dentin barrier test device using three-dimensional (3D) pulp cell cultures.

Adhesion of two bonding systems to air-abraded or bur-abraded human enamel surfaces.

Objectives: The purpose of this in vitro study was to evaluate whether mechanical alteration of the enamel surfaces with air abrasion and bur abrasion techniques could enhance the bonding performance of a three step and a self etching adhesive resin systems to enamel.

Methods: 126 extracted lower human incisor teeth were used. The teeth were divided into three groups including 40 teeth each.

Evaluation of Antibacterial Effectiveness of Desensitizers against Oral Bacteria.

Objectives: Desensitizers contribute to better clinical results by reducing the rate of cervical dentin sensitivity. However, information on their antibacterial effect is limited. This study examined the antibacterial activities of a triclosan containing (Seal & Protect), a benzalconium containing desensitizer (Micro Prime), a fluoride containing prophilaxy paste (Sultan Desensitizer), two fluoride containing varnishes (Cavity Shealth and Ultra EZ), and a dentin bonding primer (All Bond).

Microtensile and microshear bond strength of an antibacterial self-etching system to primary tooth dentin.

Objectives: The aim of this study was to test the hypothesis that the bonding ability of antibacterial bonding system to primary dentin was not different from the parental material which did not contain any antibacterial component.

Methods: Extracted human non-carious primary molars were ground to expose the coronal dentin, and then randomly divided into two experimental groups: treatment with Clearfil Protect Bond or with Clearfil SE Bond (Kuraray Medical Inc.).

Effect of a new restoration technique on fracture resistance of endodontically treated teeth.

The aim of the study was to investigate the effect of a new fiber-reinforced composite restoration technique on fracture resistance in endodontically treated premolars. Eighty sound extracted human mandibular premolars were assigned to four groups (n = 20). Group 1 did not receive any treatment.

Bond strength of two total-etching bonding systems on caries-affected and sound primary teeth dentin.

Aim: As bond strength of currently available adhesive systems in caries-affected teeth dentin on primary tooth dentin was not well known, the bond strength of two bonding systems (PQI and OptiBond Solo Plus) was evaluated on caries-affected and sound primary molar tooth dentin and observed the micromorphology of the adhesive-dentin interfaces.

Methods: By grinding both the sound (n = 30) and caries-affected (n = 30) approximal surfaces of teeth, flat dentin surfaces were obtained. The prepared surfaces were bonded with one of the each adhesive systems and a composite resin.

Effect of storage duration/solution on microshear bond strength of composite to enamel.

The aim of this study was to determine the effect of three storage solutions and two storage durations on microshear bond strength (microSBS) of a resin composite. Sixty non-carious human permanent molars were stored in three storage solutions (0.1% thymol, 10% formalin, and distilled water).

Cytotoxicity of mouthrinses on epithelial cells by micronucleus test.

Objectives: To determine the cytotoxicity of three commercial mouthrinses Klorhex, Andorex and Tanflex on buccal epithelial cells using micronucleus (MN) test.

Materials And Methods: 28 patients with aged 16-24 undergone three mouthrinses' application were analyzed before and after one week exposure. Physiologic saline was used for the control group.

Shear bond strengths of self-etching adhesives to caries-affected dentin on the gingival wall.

The purpose of this study was to evaluate the bonding ability of five current self-etching adhesives to caries-affected dentin on the gingival wall. Seventy extracted human molars with approximal dentin caries were employed in this study. In order to obtain caries-affected dentin on the gingival wall, grinding was performed under running water.

Effect of tooth age on microtensile bond strength of two fluoride-releasing bonding agents.

Purpose: The purpose of this study was to evaluate the effect of age of dentin and enamel on the microtensile bond strengths of a glass-ionomer based, all-in-one, single-step, self-etching adhesive system (Reactmer Bond, RB) and an antibacterial, two-step self-etching primer system (ABF Bond, ABF).

Materials And Methods: Extracted molars from 3 age groups (20 to 25, 35 to 40, and 50 to 55 years) were used. Enamel was removed from the occlusal surfaces of the teeth, and the entire flat surfaces were covered with composite resin (4 mm in height) following application of bonding agents.

The quantitative evaluation of apical sealing of four endodontic sealers.

The aim of this in vitro study was to evaluate the apical seal obtained with four root canal sealers: Rocanal 2, Sealapex, AH Plus, and RC Sealer. Forty root canals were prepared using the step-back technique. The specimens were divided into four groups of 10 samples and obturated by laterally condensed gutta-percha with one of the tested sealers.

Apical leakage of resin based root canal sealers with a new computerized fluid filtration meter.

In this in vitro study, the apical leakage of three root-canal sealers: AH Plus, Diaket, and EndoREZ was evaluated using a new computerized fluid filtration meter. Forty-five extracted human premolar teeth with single root and canal were used. The coronal part of each tooth was removed and the root canals were prepared using GT Rotary files and crown-down technique.

Microtensile bond strength of two resin composite materials placed with direct and indirect techniques under simulated pulpal pressure.

Purpose: The aim of this in vitro study was to compare the dentin bond strength of directly and indirectly inserted composite restorations.

Materials And Methods: Twenty extracted human mandibular molars were prepared to form a flat occlusal dentin surface using an Isomet saw under water. Five-mm-thick composite restorations (Filtek Z 250 and Clearfil AP-X) were placed on the dentin surfaces by direct or indirect techniques.

The effect on shear bond strength of different antimicrobial agents after acid etching.

The aim of the present study was to determine whether the application of different primers containing antibacterial agents (Micro Prime, Seal&Protect, and Gluma Desensitizer) can affect the shear bond strength (SBS) of an orthodontic resin composite. Seventy-two crowns of extracted lower human incisors were mounted in acrylic resin leaving the buccal surface of the crowns parallel to the base of the moulds. The teeth were randomly distributed into three experimental and one control group, each containing 18 teeth.